| Reactive oxygen species （ROS） are constantly produced in the oxidation metabolicprocess of organism. ROS is an important factor in cellular injury and the aging process.Superfluous ROS and their derivatives may cause a number of diseases. As a result, thedetection of ROS has gained much attention. Hydroxyl radical （·OH） was the most typicalreactive oxygen species. Many methods for detecting and scavenging hydroxyl radical havebeen developed, flourescence analysis obtains the favor because of its advantage of highsensitivity, good selectivity and simple operation. Therefore, it’s significant to develop spectro-fluorimetric method which is more sensitive and more selective to detect hydroxyl radical.Mercury was one of the most toxic heavy metal. It can lead to serious environmental andhealthy problems. Hence, it is important to develope new methods with more effective, rapid,low cost and easily applicable for detecting mercury ion. Rhodamine dyes have excellentphotophyical and photochemical properties such as high molar extinction coefficient, highfluorescence quantum yields. So, it is necessary to develope the application of Rhodaminedyes as fluorescent probe in mercury ion determination.In this paper, three fluorescent probes were synthesized and their selectivity for·OH andHg2+were studied. The following aspects were studied in this paper:（1） A new fluorescent probe reagent-[3-methoxy-4-hydroxyphenylmenthylidene]-anilinewas synthesized and was characterized by elemental analysis, IR and1HNMR. Combinationof Fenton reaction, a new fluorescence quenching method for determination of hydroxylradical （·OH） was proposed. The linear range and detection limit are2×10-6-2×10-5mol·L-1and8×10-7mol·L-1respectively. The spectro-fluorometric method was applied to determine the scavengingeffect of several soybeans. The results were satisfactory.（2） A new fluorescent reagent, rhodamine B hydrazide-cinnamic acid thiourea, wassynthesized and was characterized by elemental analysis, IR and1HNMR. The fluorescentprobe can react with Hg2+to produce strong fluorescent. Based on this reaction, aspectrofluorometric method was developed to determine the content of Hg2+. The liner rangeand detection limit of the method were8.0×10-7-6.0×10-6mol·L-1and7.0×10-8mol·L-1,respectively. The method was simple, selective and sensitive. It was successfully applied tothe determination of Hg2+in both tap water and river water.（3） Because of the strong thiophilic affinity of Hg2+, the probe thiooxorhodamine B- salicylaldehyde usions hydrazone was synthesized andwas characterized by elementalanalysis, IR and1HNMR. The compound can effectively connect with mercury ion anddestroy rhodamine ring structure. Mercury combination with probe molecular, the solutionhas the intense fluorescence. The liner range and detection limit of the method were1.0×10-8-1.0×10-6mol·L-1and5.4×10-9mol·L-1respectively. It was successfully applied to thedetermination of Hg2+in both tap water and river water. |
PDF Full Text Request