Tetramethylpyrazine Ameliorates Podocyte Injury Induced By The Terminal Complement Factor C5b-9in Vitro

objective: To explore the protective effects ofTetramethylpyrazine on podocytes injury induced by sublyticcomplement complex C5b-9. Methods: The cultured mouse podocytecell line(MPC5) were induced to propagate by INF-γ at33℃, whichwere subsequently cultured at37℃for differentiation for two weeks.Terminal complement complex C5b-9was formed on podocytes usingpurified C5b6and human serum,which offered C7～C9. Complementlysis was determined by measuring the release of lactatedehydrogenase(LDH). C5b-9deposition on the membrane of podocytewas detected by fluorescence microscope. The effect ofTetramethylpyrazine(TMP) on the proliferation of MPCs was observedby MTT method.The podocytes were treated with TMP before beeninjuried by C5b-9,and then F-actin and nephrin were observed byfluorescence microscopy. The reactive oxygen species(ROS) wasobserved the effect of TMP on generation. Results: Intact C5b-9wasformed on the surface of podocyte as detected by imunofluorescence andLDH release text ensured the optimum of C5b6. Sublytic C5b-9couldinduce marked F-actin cytoskeleton alteration in a time-dependentmanner．And also nephrin distribution can be observed if extended the time of C5b-9injury. C5b-9significantly increased the ROS generation inpodocyte. When podocytes were treated with TMP, the generation of ROSwas significantly decreased and cytoskeleton alteration attenuated.Conclusion: TMP ameliorates the podocyte injury induced by C5b-9partially by antioxidant pathway.