A Preliminary Study Of Granulocyte Colony-Stimulating Factor On Therapy Of Thin Endometrium Of Rat

OBJECTIVETo investigate the effect and potential mechanism of granulocyte colony-stimulating factor (G-CSF) on thin endometrium model initially.METHOD36sexually mature female virginal Sprague Dawley(SD) rats with normal estrous cycles were selected, thin endometrium models were established by perfusing anhydrous alcohol into uterine cavities for5minutes, then they were randomly divided into four groups:(1)Acute endometrial injury+G-CSF group:G-CSF (40μg/kg/d) was injected subcutaneously after the model completed every24hours for5days.(2) Acute endometrial injury+control group:same dose physiological saline was injected subcutaneously after the model completed every24hours for5days.(3)Chronic endometrial injury+G-CSF group:G-CSF(40μg/kg/d) was injected subcutaneously three estrous cycles after the model completed every24hours for5days.(4)Chronic endometrial m injury+control group:same dose physiological saline was injected subcutaneo-usly three estrous cycles after the model completed every24hours for5days. All rats were killed at the fourth estrus since the injection. Taking bilateral uterine tissue for histological examination, we observed their endometrial morphology changes and measured the endometrial thickness by hematoxylin-eosin staining (HE). At the same time we detected their cytokeratin, vimentin expression per unit endometrium area using immunohistochemistry technique.RESULTS 1. The endometrial morphology of both acute and chronic endometrial damage+control group presents thin endometrium. Compared with the control group, the endometrial thickness were thicker obviously in acute endometrial injury+G-CSF group. The endometrial lining emerge wave, glandular and luminal epithelia were intact as a cubic form, the glands were sparse and dilated partly. Stroma loose and new born capillary vessels were increased obviously. In the chronic endometrial injury+G-CSF group, the endometria were thicker, but not as much as that in acute endometrial injury+the G-CSF group, endometrial lining appears slightly wave and the number of the glands have barely changed. Stroma loose and new born capillary vessels were increased.2. The endometrial thickness of four groups were as follows:569.37±37.01μm、234.96■37.82μm、487.78■19.48μm、231.21■17.81μm. The thicknesses of each G-CSF group were significantly larger than corresponding control group (P<0.01), and the endometria of the acute endometrial injury+the G-CSF group were significantly thicker than the chronic endometrial injury+G-CSF group (P<0.01), but there was no significant difference between acute and chronic endometrium damage+control group (P>0.05)3. Cytokeratin specifically presents in the cytoplasm of the epithelial cells, representing epithelial growth. The cytokeratin area per unit endometrium area of four groups were as follows:12.57±0.40%,12.25±0.52%,12.28±0.74%,11.93±0.94%, and there was no difference between each group (P>0.05)4. Vimentin present in stroma cells and a small amout of endothelial cells, representing stroma growth. The vimentin area per unit endometrium area of four groups were as follows:13.40±0.63%, 7.27±0.48%,12.86±0.71%,7.30±0.53%. The expression of each G-CSF group were significantly larger than corresponding control group (P<0.01), and the expression of the acute endometrial injury+the G-CSF group were significantly larger than that of the chronic endometrial injury+G-CSF group (P<0.05), but there was no difference between acute and chronic endometrium damage+control group (P>0.05)CONCLUSIONS1.28-35days (seven estrous cycles) after seven estrous cycles perfusing uterine cavities with anhydrous alcohol for5minutes, thin endometrium still were presented. The stability and the replicability of the models were further confirmed.2. Granulocyte colony-stimulating factor(G-CSF) have effect on thin endometrim model to some extent, no matter the acute endometrial injury or the chronic endometrial injury, but more obvious in the acute endometrial injury+G-CSF group, probably related to the increase of stroma cell and endothelial cell.