EphA2Regulates The Expression Of Vegf Via P38Mapk Signaling Pathway

Objective:Squamous cell carcinoma of the head and neck (SCCHN) is a commom malignant tumor of head and neck. Its occurrence, growth, metastasis are dependent on angiogenesis, and tumor induced angiogenesis directly affects their invasive ability. The study of Head and neck squamous cell carcinoma angiogenesis can to some extent understand its growth, invasion and metastasis mechanism, and seek better therapeutic target. Our preliminary experiments in vivo showed that, down-expression of EphA2significantly reduced angiogenesis in head and neck squamous cell carcinoma, closely related with VEGF-reduced. Have been reported in the literature, p38MAPK signaling pathways could affect the expression of VEGF.This study is intended to clarify EphA2can regulate the expression of VEGF via p38MAPK signaling pathway in the head and neck squamous cell carcinoma in vitro experiments.Methods:(1)EphA2plasmid cDNA transfected Tu686cells and Western blot was applied to assess the EphA2protein expression;(2) Western blot and ELISA were applied to assess the expression of p38MAPK and VEGF;(3) p38MAPK inhibitor SB203580at different concentrations were used and then to detect the expression of p38MAPK and VEGF.Results:(1)EphA2overexpressed in Tu686cells transfected;(2)VEGF and phosphorylation of p38MAPK overexpressed following the overespression of EphA2.(3)VEGF and phosphorylation of p38MAPK downexpressed with the dose of p38MAPK inhibitor SB203580increased.Conclusions:(1)EphA2regulates the expression of VEGF in head and neck squamous cell carcinoma cell line Tu686.(2) EphA2regulates p38MAPK signaling pathway.(3) EphA2regulates the expression of VEGF via p38MAPK signaling pathway.