The Quantitive Study Of Tumor Associated Macrophages In Cancerogenesis Of Oral Submucous Fibrosis

ObjectiveTo investigate the express of tumor associated macrophages in oral normal tissue and lesions as well as the possible role of tumor associated macrophages in cancerogenesis of oral submucous fibrosis。MethodsCollect the data of different patients who had been diagnosed as oral submucous fibrosis and (or) oral squamous cell carcinoma and normal tissue by pathological department, in which there are30cases of oral squamous cell carcinoma with oral submucous fibrosis,20cases of oral squamous cell carcinoma without oral submucous fibrosis,20cases of oral submucous fibrosis and15normal tissue。Using the method of immunohistochemical staining to detect the expression of tumor associated macrophages。After taking photos of the slides, using Image-Pro Plus6.0to analyse the pictures and measure the data。When taking the photos, first find five regions where the positive expression is high in low power, then switch to high power, take photos and use Image-Pro Plus to count the number of macrophages and the value of integrated optical density。 The data was analyzed with SPSS11.0for windows software package, according to the conditions of levene’s test of variance homogeneity, the data between different groups was analysed with the method of Kruskal-Wallis H and One-Way ANOVA test, the multiple comparison is through Nemenyi test and SNK test, as for the data between different categories in cancer group, Mann-Whitney U test is utilized。The difference limited to P<0.05is considered statistically significant.Results1. The particles of CD68molecules is yellow or brown which locate on the membrane or the plasma, CD68positive macrophages have brown particles and irregular shape。For the oral submucous fibrosis and normal tissue, macrophages are located in the submucous layer and subcutaneous tissue, while for tumor, macrophages are usually located in the nest or paracancerous tissue2. The number and value of integrated optical in the group of oral squamous cell carcinoma with OSF、oral squamous cell carcinoma without OSF、oral submucous fibrosis、and oral normal tissue is statistically significant, which indicates tumor associated macrophages play an important role in the cancerogenesis of OSF。3. The difference between the number of macrophages of clinical stage Ⅰ～Ⅱ and Ⅲ～Ⅳ、 without and with lymph node metastasis is statistically significant, which shows tumor associated macrophages have relation with the lymph node metastasis and tumor invasion。Conclusion1. Tumor associated macrophages are widely expressed in the oral normal tissue and oral lesions。For the oral submucous fibrosis and normal tissue, macrophages are located in the submucous layer and subcutaneous tissue, while for tumor, macrophages are usually located in the nest or paracancerous tissue。2. Tumor associated macrophages in oral normal tissue、oral submucous fibrosis、oral squamous cell carcinoma without OSF、oral squamous cell carcinoma accompanied by oral submucous fibrosis increase gradually, which indicates tumor associated macrophages participate in cancerogenesis of oral submucous fibrosis。3. Tumor associated macrophages have difference in different lymph node metastasis and clinical stages, which show it have relation with the metastasis of lymph node and invasion of oral squamous cell carcinoma。...