Investigation Of The Endoplasmic Reticulum Stress In Doxorubicin-induced Cardiotoxicity

Objectives:To study the expression of protein CHOP/GADD153in doxorubicin-induced cardiotoxicity in H9C2myocardial cells and explore the correlation of endoplasmic reticulum stress to doxorubicin-induced cardiotoxicity, in order to investigate a new mechanism in doxorubicin-induced cardiotoxocity.Methods:H9C2cells cultured in vitro within logarithmic growth phase were used to study. Study one was that the H9c2cells were exposed to DOX for12hours at the concentrations of2,5,and10umol/l respectively.Sduty two was that the H9c2cells were exposed to DOX at the concentration of5umol/l for6,12,and24hours respectively. The expression of CHOP of the control group,different time group and different concentrations group were measured with Western blot analysis.Statistical analysis:Statistical analysis was performed with the SPSS statistical software, SPSS PASW Statistics. Experimental datas were represented by mean±tandard deviation (X±D).Measurement data were compared by one-way analysis of variance. A value of p<0.05was regarded as significant. Results:1. Part one showed that,compared with the control group,the expression of CHOP protein in doxorubicin group were significantly increased at the concentration points of2,5and10umol/l12hours after doxorubicin treated H9C2cells (p≤0.05).2. Part two showed that,compared with the control group,the expression of CHOP protein in doxorubicin group were significantly increased at the concentration of5umol/l doxorubicin for6,12and24hours (p≤0.05).Conclusions:1. Endoplasmic reticulum stress occured in a time and dose dependent manner in doxorubicin treated H9C2myocardial cells.2. Endoplasmic reticulum stress may be involved in doxorubicin induced cardiotoxicity.