Study On The MicroRNA Expression Profiles In Esophageal Squammous Cell Carcinomas

Purpose:Esophageal carcinoma is one of the most common human cancers, and it has a high incidence rate and mortality rate especially in our country. The infiltration and metastasis of esophageal carcinomas have a close relation with prognosis. We selected10cases of esophageal squamous cell carcinoma with detailed clinical-pathological data and follow-up information, to detect and analyze the differentially expressed microRNAs between lymph node metastasis group and non-metastasis group, between dead within five years group and survival group with microRNA microarray. Our study will not only play an important role in realizing the molecular mechanism of carcinogeneisis and the development of esophageal squamous cell carcinoma, but also play an academic foundation for exploring related diagnostic and prognostic biomolecular markers.Methods:10cases of fresh frozen samples of esophageal squamous cell carcinoma tissues and relevant normal tissues were selected. The total RNA was extracted with Trizol method whose concentration and purity was measured by spectrophotometer, the quality was tested by degenerative agarose gel electrophoresis before hybridization.The sample RNA was then marked with the miRCURYTM Array Power Labeling kit, then hybridized with the miRCURY LNATM microRNA chip (including1891human, rats, mice, the relative virus’microRNAs, and385human miRPlusTM probes which are not included in the miRBase yet). The hybridization results were scanned by Axon GenePix4000B chip scanner, analysed by GenePix pro V6.0software. MEV software (v4.6, TIGR) was used to do differentially expressed screening (with P value<0.05and Fold Change value≥2or≤0.5) and do cluster analysis.Results:There were448microRNAs detected expressing in all the10cases of esophageal squamous cell carcinoma tissues. There were10differentially expressed microRNAs between esophageal squamous cell carcinoma tissues and normal tissues,8of them were upregulated, and2of them were downregulated. However,40microRNAs were differentially expressed between lymph node metastasis group and non-metastasis group,22of them were upregulated, and18of them were downregulated.7microRNAs were differentially expressed between dead in five years group and survival group,4of them were upregulated, and3of them were downregulated.Conclusions:MicroRNA expression profiles microarray is an effective high-flux method to detecting differential microRNAs expression in esophageal squamous cell carcinomas. The differently expressed microRNAs detected in the microRNA microarray analysis, may be new and characteristic molecular markers of the squamous cell carcinomas of esophagus, and could help us get better understanding of the microRNAs’ functions in the carcinogeneisis and development of esophageal squamous cell carcinomas, and go further in searching for related biomolecular markers of clinical diagnosis and treatment in esophageal squamous cell carcinomas.