The Exploration Of The Molecular Mechanism Of Fluid Shear Stress On The Proliferation Of Osteoblasts In Vitro Experiments

ObjectiveTo explore the most suitable value, the action time and preliminary molecular mechanism of fluid shear stress on the proliferation of osteoblasts.MethodsMC3T3-E1cells were seeded at2×105per glass on glass slides cultivated for5days and then subjected to fluid shear stress of2,6,12,20,25dynes/cm2respectively using a parallel plate flow chamber. Each magnitude of fluid flow lasted15,60,150minutes respectively. MTT and ALP were adopted to test the cell proliferation and one group with the best magnitude of FSS and length of time were chosen to conduct microarray analysis.ResultsMC3T3-E1cells subjected to12dynes/cm2with the duration of60minutes exhibited a higher proliferation and ALP activity, and the analysis of microarray indicated that about92elevated and89depressed genes directly and indirectly participated in the biological activities of osteoblasts under the influence of fluid shear stress, in which APH1B of Notch signal pathway was obviously elevated and DDX58of RIG-I-like receptor signaling pathway was depressed.ConclusionFluid shear stress elicited stimulative and protective effect on the proliferation of MC3T3-E1cells through multiple signal pathways involving Notch and RIG-I-like receptor signal pathway.