Prulifloxacin Complex Fluorescent System And Its Application

The quinolones (pyridone acids) are a family of synthesized antibiotics, which are named because of the quinolone structure in the organic compound. The number of drug varieties has increased greatly since the first quinolones drug,nalidixic acid discovered in1962. The action mechanism of fluoroquinolone drugs is inhibiting bacterial DNA helix enzyme and (or) topisomerase IV, affecting the normal morphology and function of the bacterial, impeding the normal DNA replication, transit and reorganization, and which enable they have resulting in the rapid germicidal effect. The quinolones which are applied widely in clinic, have many advantages such as broad antibiotical spectrum, good oral absorption, high plasma concentration, less drug-resistant strains, rapid distribution to various organizations and long half-time, and etc.The quinolones can form complex with rare earth ions in certain conditions. The complex absorbed ultraviolet light energy,emited the characteristic fluorescence of the core ion via inter-system crossing. The fluorescence spectroscopy of rare earth ions is a sharp lines spectrum, with high selectivity. Its luminous intensity will be greatly enhanced when combined with appropriate organic ligands. The research about the fluorescence charactistics and the application of the complex has developed greatly in recent years. There is considerable interest in the study on these complexes because of their potential application for determination of the rare earth elements, or conversely, for determination of the organic ligands, or as fluorescent probes in clinical chemistry, molecular biology, environmental sciences, and etc.In this thesis, the fluorescence characteristics of the PUFX-Eu3+complex are studied. The fluorescence system is optimized by introducing of surfactant. The above fluorescence system is applied to determine the PUFX in pharmaceutical samples and its active metabolite in biological samples with satisfactory results. And the sensitization mechanism of these systems is also studied by sereral measures.The first chapter introduced the basic principles and research progress of the lanthanide organic complexes and lanthanide ions fluorescence probe method, and overviewed the analytical research progress of the quinolone drugs.In the second chapter, the PUFX-Eu3+fluorescence system is studied in detail.It is found that the anionic surfactant SDBS can greatly increase the fluorescence of the system(27-fold).The PUFX-Eu3+-SDBS fluorescence system is set up. The excitation and emission wavelengths are determined as274nm and616nm, respectively. Under the optimum conditions, a linear relationship is obtained between the fluorescenceintensity and europium ion concentration in the range of2.5×10-7～2.0×10-6mol·L-1.The correlation coefficient(r) is equal to0.9991and the detection limit of PUFX is5.00×10-10mol·L-1. The method is successfully applied to the determination of PUFX in pharmaceutical samples. The mechanism of fluorescence system is studiedIn the third chapter, the UFX-Eu3+fluorescence system is studied in detail. It is found that the anionic surfactant SDBS can greatly increase the fluorescence of the system(37-fold). The excitation and emission wavelengths are determined as276nm and616nm, respectively. Under the optimum conditions, a linear relationship is obtained between the fluorescence imensity and europium ion concentration in the range of5.0×10-8～2.0×10-6mol·L-1. The correlation coefficient(r) is equal to0.9990and the detection limit of UFX is2.0×10-10mol·L-1. The method was successfully applied to the determination of UFX in human urine/sermn samples. In addition, we also studied the luminescence mechanism of the system through fluorescence spectra,ultraviolet absorption spectra, surface tension, and so forth.In the last chapter, a new method of sensitized fluorescence spectrometry using the micellar system for the trace analysis of PUFX has been developed. The experiments indicate that sodium dodecyl sulfate (SDS) can enhance greatly the fluorescence signal of PUFX. The excitation and emission wavelengths are determined as275nm and420nm, respectively. Under the optimum conditions, a linear relationship is obtained between the fluorescence intensity and europium ion concentration in the range of1.0×10-10～1.0×10-8mol·L-1.The correlation coefficient(r) is equal to0.9974and the detection limit of PUFX is5.0×10-12mol·L-1. The method is successfully applied to the determination of PUFX in pharmaceutical samples.The main characteristics of this thesis are as follows:1. The new PUFX-Eu3+-SDBS and UFX-Eu3+-SDBS fluorescence system are set up and used for determination of UFX in biological samples and PUFX in pharmaceutical samples.2. The new PUFX-SDS fluorescence system are set up and used for determination of PUFX in pharmaceutical samples.3. The luminescence mechanism of these systems were studied in detail through ultraviolet absorption spectra,surface tension and so forth.The above research work had potential application value to the lanthanide analysis, biological and pharmaceutical analysis, and offered certain theoretical and experimental basis for the correlative research of lanthanide_quinolone complexes.