The Expression Of CXCL16、INOS In EAE Rats’ Spinal Cord And The Effect Of Edaravone

ObjectiveTo observe the expression of CXC chemokine ligand16(CXCL16) and induciblenitro oxide synthase (iNOS) in the spinal cord of the experimental autoimmuneencephalomyelitis(EAE) rats model, and study the intervention mechanism ofedaravone in EAE and the effect of CXCL16and iNOS. So as to provide new idea forclinical treatment of multiple sclerosis(MS).Methods72female Wistar rats were divided into four groups by randomizationmethod:normal group(N group),EAE group,low-dose group(LDE group),high-dosegroup (HDE group).Subcutaneous inject the antigen which include complete Freund’sadjuvant(CFA) and guinea pig spinal cord homogenate(GPSCH) per0.5ml induceEAE model in rats. After immunization, each rat in low-dose group inject edaravoneinjection3mg/kg·d daily,each rat in high-dose group inject edaravone injection10mg/kg·d daily and record the neurological score of all the rat every day. Each groupkill6rats on the7th day、14th day、21st day.Take the lumbar enlargement of spinalcord for HE staining and immunohistochemical staining.Observe the inflammatory cells,expression of CXCL16,expression of iNOS in the spinal cord.ResultsThere’s no ill rat in normal group.The incidence rate of EAE group was77.78%.Either the low-dose group’s incidence rate or the high-dose group’s incidencerate was significantly lower than the EAE group’s（P＜0.05）.The neurological score ofLDE group and HDE group were lower than EAE group（P＜0.05）,the neurologicalscore of HDE was less than LDE group（P＜0.05）.The inflammatory cells, the positiveexpression of CXCL16and iNOS were found in EAE、LDE and HDE group, and consistent with the severity of disease. In the14th day, the number of inflammatorycells、CXCL16-positive cells、iNOS-positive cells in the LDE、HDE group weresignificantly less than the EAE group（P＜0.05）, and every index of HDE group wasless than LDE group（P＜0.05）. In the21st day, the number of inflammatory cells、CXCL16-positive cells、iNOS-positive cells in the HDE group was significantly lessthan the EAE group（P＜0.05）.Conclusion1. The CXCL16and iNOS express in the EAE rats related with disease severity.2. The intervention of edaravone can reduce the incidence of EAE rats,also canimprove the clinical symptoms related with the dosage.3.The protective effect of edaravone in EAE is its free radical scavenging,reducing inflammation, reducing CXCL16and iNOS expression.