Enhancing DDP Effect With NOC On The Growth Inhibition And Apoptosis Of Human Ovarian Cancer Cell-8910

AIM:To study the enhancing effect of the conventional chemotherapy drugs DDP with NOC on the growth inhibition and apoptosis of human ovarian cancer cells HO-8910,and to investigate if it include the incrising expression of P38MAPK and P-P38MAP, the Glutathione depletion and the caspase-3activation, and to provide experimental evidence and theoretical basis for the NOC as the development of clinical ovarian cancer chemosensitizer.METHODS:In vitro of human ovarian cancer HO-8910was the object of study.then Cisplatin and8-nitro chrysin interfere with ovarian cancer HO-8910cells.MTT assay was detected after treatment in each group HO-8910cell proliferation inhibition; Apoptosis rate of the each treatmenin group HO-8910cells was measured by FCM(flow cytometry)staining with AnnexinV-FITC/PI;P38MAPK and P-P38MAPK protein level of expression in each group HO-8910cells were observed after treatment by Westem-Blot; Spectrophotometry were used to detect GSH content changes and caspase-3activity changes in the treated group.RESULTS:MTT assay results showed that compared with the control group, each dose DDP,NOC inhibited the growth of HO-8910cells, and the effect were dose and time dependent.2μmol.L-1DDP+20μmol.L-1NOC joint administration of cell growth inhibition rate of71.8%almost reach to the single20umol.L-1DDP group of cell growth inhibition rate of74.1%.(P>0.05) It showed that NOC and DDP combined to produce the equivalent of10times the dose of DDP on the HO-8910cells proliferation inhibitory effection. The results of the AnnexinV-FITC/PI double staining flow cytometry showed that:after48 hours’ effection with NOC and DDP on HO-8910cells,2μmol..L-1DDP+20μmol.L-1NOC combined administration group apoptosis rate was43.5%, almost as monotherapy group20μmol.L-1DDP apoptosis rate of47.7%.(P>0.05). This demenstrated that NOC combined with low doses of DDP could synergisticly induce apoptosis in ovarian cancer cells apparently. Western results showed:Compared with the control group, the expression of P38MAPK and P-P38MAPK increased in the treated groups(P<0.05), and NOC and DDP combination group was as the same as DDP alone treatment group(P>0.05); Spectrophotometric test results showed compared with the control group, the GSH content of each treatment group decreased(P<0.01); Caspase-3test results showed:compared with the control group, Caspase-3activity of the treated groups gradually increased.(P<0.01)CONCLUSION:1.The8-nitrorchrysin can enhance the growth and proliferation inhibitory effect of cisplatin in human ovarian cancer HO-8910cells.2. The8-nitrochrysin can promote cisplatin induced HO-8910cells apoptosis.3. The8-nitrochrysin chemotherapy sensitivity may be related to the depletion of GSH, caspase-3activity increased, and promoting P38MAPK and P-P38MAPK protein expression.