The Effect Of DHA On Growth And Proliferation In MGC803Cell

ObjectivesDiscuss the effect and its mechanism of DHA in the growth and proliferation of thegastric cancer cell MGC-803, provide experimental basis for the development of the newantitumor drugs.Methods1Culture human gastric carcer cell MGC-803in vitro,1、10、30、60、100uMol/L of DHA treated on human gastric cancer cell MGC-803respectively, MTT assay wasused to analyse the stuation on the growth and proliferation of MGC-803cell.2Treated the cell with60uMol/L of DHA, preparing the cell slide, immunocytoche-mical staining was used to observe the expression and distribution of β-catenin proteinin gastric cancer cell MGC-803.3Western blotting was used to analyze expression of GSK-3β, phosphorylationGSK-3β,β-catenin protein in gastric cancer cell MGC-803treated with DHA at sameconcentrations with the prolongation, as well as the phosphorylation of GSK3β ingastric cancer cell MGC-803treated with GSK-3β inhibitor Licl pretreated with DHA.Results1The MTT assay showed that different concentrations of DHA can inhibit the growthand proliferation of MGC-803cell differently, the inhibition rate was30.43%、58.44%、64.52%respectively（P<0.05） after treatment with DHA,and the inhibitory effectconcentrations of DHA at30、60、100（uMol/L）, and the50%inhibition rate of DHAis60uMol/L.2The Immunocytochemistry results showed that the expression of β-cateninprotein in MGC-803gastric cancer cells treated by DHA is mainly distributed in themembrane and cytoplasm, compared with the control group, β-catenin total reduced by 22.43±0.66%(P <0.05).3Western-blot analysis indicated that treated with DHA at60uMol/L for0.5、1、3、6h, the protein level of β-catenin was up-regulated, while the GSK-3β and P-GSK-3β was down-regulated. Moreover treated MGC-803cell with Licl, the protein level ofβ-catenin is reducted by32.19±2.32%comparised with the control group, treated DHAwith24h, the protein level of β-catenin is reducted by42.54±3.21%comparised withthe control group, the β-catenin protein increased by10.33±2.21%of the group treatedwith Licl comparised with the group treated with DHA(P<0.05).Conclusion1. DHAcan inhibit the growth and proliferation of MGC-803cell effectively.2.DHA inhibit the growth and proliferation of MGC-803cell probably by reducethe level of phosphorylation of GSK3protein which expressed in MGC-803cell,increasing the activation of GSK-3β, and promoting the degradation of β-catenin.