| It is more common that the mandibular is abnormal in orthodontictreatment. The excessive mandibular will lead to Ⅲ malocclusion, Theinsufficient mandibular will lead to Ⅱ malocclusion,Malocclusion willseriously impact on the patients face of coordination，it will also causeserious adverse effects on the patient’s chewing function, facialmorphology，Temporomandibular disorders and the psychological. Itsetiology is complex，osteal malocclusion occurs through different channelsto regulate the impact of a variety of factors, the growth of the mandible isnot only subject to the regulation of systemic factors, and feedback effectsof local growth factor, which also occupy an important position, theadjustment process is more complicated. Dmp-l knockout mouse modelshow that the mandibular and condylar cartilage was reduced. Research hasshown that Dmp-1may be one of the important gene in the regulation ofmandibular and condylar cartilage development.Dentin matrix protein1is high in phosphorylation extracellularmatrix，it belong to members of the family of SIBLINGs. The expression ofDmp-1is higher in bone tissue and condylar cartilage in the meantime. Research has shown that Dmp-1may be one of the important gene in theregulation of mandibular and condylar cartilage development. ut theexpression of Dmp-1in Class II and Ⅲ malocclusion which caused bymandibular dysplasia has not been reported. Explore the expression ofDmp-1in mandibular, which is helpful to the diagnosis and treatment ofmandibular growth defect in the wrong tooth and deformity.Objective:The experiment through detecting the expression of Dmp-1mandibulargrowth mandibular growth abnormalities caused by skeletal II, IIImalocclusion patients and their expression in the mandible normaldevelopment stages of blood,to explore the relationship of the Dmp-1expression and growth of the mandible.Malformation diagnosis formandibular bone abnormalities caused by the wrong tooth together toprovide more rapid and accurate diagnostic indicators provide a basis fororthodontic program development.Methods:(1) This experiment selects Ⅰ, Ⅱ, Ⅲmalocclusion36cases each,classⅠas control group, classⅡ, Ⅲfor the experimental group. Accordingto improved cervical analysis bone age growth stage is divided into peak(CVS3period) the experimental group and control group, growth peak later(CVS4) the experimental group and control group, for a total of six groups.Take the fresh blood samples. (2) By RT-PCR were used to detect the expression of BMP-4mRNAblood from patients in each experimental group.(3)Western-blot analysis were used to detect the expression of BMP-4protein in the blood of patients in each experimental group.Result:(1) In CVS3group, by RT-PCR results showed that the bone class Ⅰmalocclusion deformity group expression of Dmp-1mRNA：5.5931±1.2761μg/μl, skeletal Class II malocclusion group, the expressionof Dmp-1mRNA：3.5735±2.2683μg/μl，skeletal Class Ⅲ malocclusiongroup, the expression of Dmp-1mRNA：10.4310±1.0447μg μl.There wassignificant deviation between Class Ⅲ and class Ⅰ malocclusiongroups(P＜0.05), but there was no significant difference between the ClassⅡ and Class Ⅰ malocclusion group(P＞0.05).In CVS4group, by RT-PCR results showed that in skeletal Class Ⅲmalocclusion group the expression of Dmp-1mRNA is11.9214±2.1761μg μl, in ClassⅠmalocclusion group which is7.0763±1.7315μg/μl. There was significant deviation between the twogroup(P＜0.05).The expression of Dmp-1mRNA in skeletal Class Ⅱmalocclusion group is4.3206±1.8630μg μl. There was significant deviationbetween Class Ⅲ and class Ⅰ malocclusion groups(P＜0.05), but therewas no significant difference between the Class Ⅱ and Class Ⅰmalocclusion group(P＞0.05). (2) In CVS3group,the result of WB method showed that theexpression of Dmp-1in Class Ⅰ malocclusion group is15.4064±2.9312μg μl,which is12.5718±3.5302μg μl inClassⅡmalocclusion group.The expression of Dmp-1in Class Ⅲmalocclusion group is24.2109±4.4261μg μl. There was significantdeviation between Class Ⅲ and class Ⅰ malocclusion groups(P＜0.05),but there was no significant difference between the Class Ⅱ and Class Ⅰmalocclusion group(P＞0.05).In CVS4group, the result of WB method showed that the expressionof Dmp-1in Class Ⅲ malocclusion group is37.0534±3.7611μg l,ClassⅠmalocclusion group which is27.1705±2.7813μg/l. There was significant deviation between the twogroup(P＜0.05).The expression of Dmp-1in Class Ⅱ malocclusion groupis21.3025±2.0710μg/l.There was significant deviation between Class Ⅲand class Ⅰ malocclusion groups(P＜0.05), but there was no significantdifference between the Class Ⅱ and Class Ⅰ malocclusion group(P＞0.05).(3)WB results of the experimental group could produce immuneresponse with the Dmp-1antibody,showing the bands in the PVDFmembrane,but the intensity of signal expression is difference.Conclusions:In the both groups,the expression of Dmp-1in Class Ⅰmalocclusion group is significantly lower than that in ClassⅢmalocclusion group,suggesting that Dmp-1may promote the growth of the mandible.In the both groups,the expression of Dmp-1in Class Ⅱ malocclusiongroup is lower than that in ClassⅠmalocclusion group,suggesting thatDmp-1may promote the growth of the mandible. |
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