Neural Restoration And Mobilization Of Bone Marrow Stem Cells In Focal Cerebral Ischemia Stroke Rats

ObjectiveTo investigate the effects and mechanism of granulocyte-colonystimulating factor (G-CSF) on mobilization of bone marrow stem cells and onits recruitment to the infarct brain tissues of focal cerebral ischemia rat.MethodMCAO model was induced by using the filament occlusion method.60successful MCAO model rats were randomly divided into control group andG-CSF treated group. Treated group were subcutaneously injected with G-CSF(2,10,50,250μg/(kg·d)), for1,3,5,7days.Neurological scale were evaluatedand the number of mononuclearcyte in peripheral blood were counted, andCD31and CXCR4mRNA were detected by RT-PCR. Brain tissue were takenafter perfusion fixation and sections were cut to check the expression ofCD31、CD133and SDF-1by immunohi-stochemistry.ResultsCompared with the control group, Neurological function of G-CSF treatedgroup improved (P <0.05).Number of mononuclear cells in peripheral blood increased significantly in G-CSF group; in the low-dose of G-CSF group effects(2,10μ g/(kg·d)) mRNA expression of CD31, and CXCR4of MNC increasedcompelled with the extension of time. In high dose (50,250μ g/(kg·d)) group,CD31and CXCR4mRNA increased with dosage increase in3days，butgradually weakened with the time extension to7days; Expression of CD31、CD133and SDF-1in brain tissue after G-CSF treatment detected by ICH wassignificantly increase.