The Expression And Significance Of Calretinin In The Colon Of Patients With Hirschsprung’s Disease

Objective:To detect the expression of calretinin in different segments ofthe colons of patients with Hirschsprung’s disease(HD), explored itsfeasibility in the diagnosis of HD (especially newborns HD).Methods:1．The experimental group specimens were collected from50HD patients in Medical University Of Chongqing Affiliated Children’sHospital from2010to2011．in this group <1months six cases,1-3months23cases,4months-1year-old11cases,>1year old10cases,Thenormal control group specimens were collected from10age—matchedpatients without HD．2． Fi rst， All aganglionic bowel segments andganglionic segments specimens of50HD patients and normal control groupspecimens were underwent haematoxylin and eosin(HE)staining andscanned, then CR immunostaining was used to the same specimens.Finally, Two staining results.were made analysis by image analysisprogram(Image-Pro—Plus) and SPSS17.0data analysis system forstatistical analysis.Results:1.There was a significant difference in the expression ofcalretinin mean optical density between HD spastic segments group andecstatic segments group, normal control group (P<0.01) both in submucosalplexus and Myenteric plexus.2.Ganglion cells could be seen in HE stainedspecimens in ecstatic segments, but loss in spastic segment, CR stainingresults of immunohistochemistry are consistent with HE stainingresults.3.Newborns and older children HD spastic segment submucosalplexus and Myenteric plexus.had no significant difference in the expressionof CR (P=0.816).Conclusions:1. Rectal mucosa could be taken to observe the expressionof calretinin, to help the diagnosis of Hirschsprung’s disease.2. CRImmunohistochemical staining for the diagnosis of neonatal HD has obviousadvantages compared with traditional methods.