Analysis Of The Disinfectant Resistant Gene And The Homology In Acinetobacter Baumanii Causing Nosocomial Infection

Background:Acinetobacter baumannii is conditional pathogenic bacteria widelydistributed in nature. It normally cause infection in long-term inpatient,intensive care unit and major surgery patients, especially in the low bodyresistance or impaired immune function. At present, resistant Acinetobacterbaumannii is an important nosocomial infection pathogens. These strainsinfected patients have caused great difficulties to the clinical treatment. It isthe most concerned about hospital infection pathogens. Disinfection isextremely important in hospital infection. Studies had shown that thedisinfectant resistant gene of qacEâ–³1rate was high and closely related tothe quaternary ammonium and biguanide compounds disinfectant resistance.So detection and conduction an epidemiological study on the disinfectantresistant gene of Acinetobacter baumannii have significance.Objective:To investigate the disinfectant resistant gene of qacEâ–³1in multi-drugresistant Acinetobacter baumanii(MDRAB) causing nosocomial infection isolated from our hospital and the characterization of the positive strainhomogeneity.Methods:A total of75MDRAB were detected by PCR assay and verified by DNAsequencing. The REP-PCR assay was used for genotyping the positivestrains.Results:Among the75strains, there were33strains carrying qacEâ–³1in75strains, the positive rate was44%;25strains were pan-drug resistant;13strains carried qacE positive rate was52%. These positive strainswere divided into5genotypes by REP-PCR.29strains were classified astype A, and there was only one strain belonging to type B, type C or type D,respectively.Conclusion:The high detection rate of the disinfectant gene of qacEâ–³is1possiblyrelated to the gradual increasing MDRAB causing nosocomial infectionisolated from our hospital. And the main prevalent genotype was type A. Thestrains carrying qacEâ–³inc1l iwneedr et op odsesvieblloyp to MDRAB.