The Study Of ACE2Expression In The Hippocampus Of Diabetic Rats And Its Effect On Cognitive Function

Objective: To study the expression of angiotension-converting enzyme2(ACE2) in the hippocampus of diabetic rats and its effect on cognitivefunction.Methods: Twenty male SD rats were randomly divided into two groups:normal control group and diabetic group.Diabetic rats model wereestablished by streptozotocin, the Morris water maze test in rats learningand memory ability. The protein expression of ACE2was examined byWestern blot.The mRNA level of ACE2was examined by RT-PCR.Result: Comparing to the normal group, learning and memory level ofdiabetic group decreased transparently（P<0.05）, the protein and mRNA level of ACE2in hippocampal neuron decreased（P<0.05）.Conclusion: The decreased expression of ACE2mRNA and proteinin hippocampus of diabetic rat maybe associated with cognitivedysfuncyion. Objective: To explore the effect of ACE2on oxidative stress andcognitive function in the hippocampus of diabetic rats.Methods: Thirty SD male rats were meanly divided into normal group,diabetic group, ACE2treatment group randomly. Diabetic rats model wereestablished by streptozotocin. Learning and memory behaviors weremeasured using the Morris water maze test. The levels ofmalondialdehyde(MDA), superoxide dismutase(SOD) and glutathioneperoxidase(GSH-PX) were detected by colorimetric method.Result: Comparing to the normal group, learning and memory level of diabetic group decreased transparently（P<0.05）.Learning and memorylevel in the ACE2treatment group were much better than diabetic modlegroup（P<0.05）, and the ACE2treatment group had significantly higheractivities of SOD and GSH-PX, lower contents of MDA compared with thediabetic modle group（P<0.05）.Conclusion: ACE2may improve cognitive ability in diabetic rats byattenuating oxidative stress.