The Inhibition Effectes Of Ophiocordyceps Sinensis Extrcts On LPS-induced Inflammation

The study is based on the inflammation model in whichlipopolysaccharide (LPS) is used to stimulate mouse peritonealmacrophage RAW264.7. It studies the in vitro inflammation effects ofextracts from Ophiocordyceps sinensis which is proved to be the realanamorph of precious traditional Chinese herbalmedicine——Cordyceps. Meanwhile, the anti-inflammationmechanism of aqueous extract is pilot studied. This research providetheoretical and practical basement to further in vivo study, clinicalstudy, clinical medicament as well as the exploitation ofOphiocordyceps Sinensis market.In this study the cytotoxic effects of six extracts(ethanol extract,aqueous extract and fractionated extracts of ethanol extract——petroleum ether extract, chloroform extract, acetic ether extract) andthe residual on mouse peritoneal macrophage are examined by MTT ina proper concentration range. Then the impact on nitric oxide releasingis determined by Griess method. Western Blot is used to examine bothtime-effect and dose-effect of aqueous extract inducible nitric oxidesynthase(iNOS), cyclooxygenase-2(COX-2), p38in MAPK pathway andp56in NF-κB pathway in LPS-stimulated macrophage.The results show that OS extracts has no cytotoxicity onLPS-stimulated macrophage. Ethanol extract and aqueous extractcould inhibit iNOS production in dose-dependent manner inconcentrtion of10,100,250,500μg/mL. The inhibition of chloroformextract is the strongest of all the fractionated extracts in100μg/mL andit is the only extract that could inhibit NO production in10μg/mL. Intime-dependent reaserch, aqueous extract could inhibit iNOS, COX-2and p3S production most in6h,12h and15min relatively. Aqueousextract has inhibitory effect on iNOS, COX-2and p38release in dose-dependent manner in LPS-stimulated macrophge.In conclusion, OS extracts have obvious anti-inflammation effects invitro.