The Effects Of Lactosucrose On Intestinal Tight Junction And Barrier Function In Weaning-Rats

The intestinal barrier plays an important role as the first line of defense of the organism against external pathogens, because of the psychological, environmental, nutritional and other factors, Gastrointestinal structure and intestinal micro-ecological environment of weaning young animals are altered by weaning stress, undermining the intestinal barrier function, increasing intestinal permeability, causing inflammatory diarrhea, results of the normal growth and development of weaning young animals are adversely influenced, and even death. In this reseach, firstly, study the effects of different doses of Lactosucrose on the growth performance in the different periods in weaning-rats, followed by LPS stimulation, simulate the body environment of pre-sick in the weaning-rats, reseach the biochemical indicators of serum, physicochemical properties of stool, intestinal internal micro-environment and morphology, intestinal permeability, intestinal mucosal cytokine levels and epithelial cell tight junction protein expression changes in weaning-rats administrated Lactosucrose, in order to reveal the mechanism of different doses of Lactosucrose on intestinal tight junctions and barrier function in weaning animals. Besides, it also provides a reference for infant formula.Results showed that, in the second week, Lactosucrose had benefits for the growth performance of weaning-Rats, increasing trend for body weight(p>0.05), feed conversion decreased significantly(p<0.05). As opposed to positive weaning-Rats, Lactosucrose significantly increased serum IgM levels(p<0.05), a significant reduction in serum ALB(p<0.05), BUN(p<0.05), and CK(p<0.05) levels, a significant reduction of NH3-N levels in cecal chyme(p<0.05), an increase of acetic acid and monoprop content in cecal chyme, and butyric acid content in the feces(p<0.05), improved the shape of the stool score(p<0.01), reduce stool frequency(p<0.05), significantly increased jejunal and ileal villus height/crypt depth ratio(p<0.05), and the thickness of the colonic mucus layer and epithelial goblet cell number (p<0.05), a significant reduction of the intestinal permeability (p<0.05), and inhition of serum DAO activity, regulating the balance between the pro-inflammatory and anti-inflammatory cytokines, a significant increase of colonic mucosa Occludin protein expression (p<0.05).In summary, Lactosucrose had helpful for the growth performance of the weaning-Rats. Lactosucrose protected the weaning-Rats with LPS intestinal barrier function, by inhibiting the mucosa atrophy, improving the micro-environment of gut, increasing intestinal tight junction expression and reducing intestinal permeability.