Study On A Solid Monophyletic Chlorine Dioxide Disinfectant

Chlorine dioxide (ClO2) is called “the fourth generation” disinfectant withenvironmental friendship, high efficient, broad spectrum germicidal characteristicwhich gradually becomes widely used disinfectant instead of chlorine disinfectant.Nowadays, ClO2disinfectant mainly includes two kinds of preparations which isliquid and solid form. The liquid one has big volume and inconvenient fortransportation and the solid one is usually binary form which is inconvenient to use.So we are studying on a solid monophyletic chlorine dioxide disinfectant in order toprovide new ClO2disinfectant formula which is convenient for usage andtransportation without the limitation above. The study includes formula selection,killing microorganism effect and disinfection impact factor observation.Formula selection is mainly based on reaction of sodium chlorite and solid acidanhydride, and we stabilize the disinfectant with coating effect of high polymermaterial and deactivation effect of inorganic compound. In the optimization ofdisinfection formula phase, we observe two main ingredients which product ClO2,sodium chlorite and sodium hydrogen sulfate, with factorial designs. Base on the fileswith ANOVA of two factor factorial designs, we adopted SAS8.0statistical softwareto analyse the results. The results said that the effect of the sodium chlorite dose (A)and sodium hydrogen sulfate dose (B) on ClO2output has statistical significance（F=31810.53，P<0.0001；F=31577.50，P<0.0001）and the interaction of two fatorsalso has statistical significance. According to the experimental data, sodiumhypochlorite dose has more significant effect on output of ClO2due to three levels ofsodium hydrogen sulfate dose, that is to say output of ClO2is increase with sodiumchlorite dose. Meanwhile, output of ClO2reaches highest when sodium hydrogensulfate dose is40g and sodium chlorite dose is constant. According to the resultswhich is pairwise comparison of nine average values, finally we choose the formulaproducing the highest output of ClO2that is the mixture of30g sodium chlorite,40gsodium hydrogen sulfate,5g coating reagent,15g deactivator,12g desiccant. In formula ClO2can reach up to383.89mg/L in water and only reduce8.4%after3months at37℃this react.The utility model on germicidal efficacy of chlorine dioxide disinfectant hasshown a good kill, fast killing speed, desired low concentration.25mg/L ClO2disinfectant react for1min to Staphylococcus aureus (ATCC6538), Escherichia coli(8099), Candida albicans (ATCC10231), the killing rate can be up to100%.Sporicidal efficacy of25mg/L chlorine dioxide disinfectants on Bacillus subtilis var.niger (ATCC9372) was99.94%, when chlorine dioxide was increased to50mg/L for1min on Bacillus subtilis var. niger, killing rate can reach100%. The chlorine dioxidedisinfectant with50mg/L for1min, could inactivate the virus of poliomyelitis (PV-1)with mean inactivation on numerical>4.00, achieve inactivation effect. In addition,the chlorine dioxide has fast germicidal speed,12.5mg/L chlorine dioxide disinfectingeffect on Escherichia coli,1min and10min of the killing rate was99.92%. Prolongaction time failed to increase the killing rate. This may be associated with chlorinedioxide activated radicals generated during the rapid demise of. So in the process ofuse, should be done by using now.In the study of the factors influencing on germicidal effect in the process, wethrough orthogonal experiment the visual analysis method and SAS statisticalsoftware to analyze statistics. The test show, the concentration of the disinfectant, calfserum content, and temperature on germicidal efficacy of disinfectant effects weresignificant (P <0.0001).This topic through formulation, disinfection effect research, influencing factors,developed a unitary solid chlorine dioxide disinfectant, which is characterized by highactivation rate, good stability, fast germicidal speed. The utility model is a convenientprepared for chlorine dioxide disinfectant.