The Role Of Inflammatory Agents On The Liver Of Animals

Guinea pigs6, the quality between the350～420g, female, from the Institute of Experimental Animal Center of Dali. Randomly divided into three groups:A group-operated group, laparotomy, removal of part of the liver; another line of hilar block; B group, liver biopsy abdominal injection of turpentine; C group, ultrasound-guided percutaneous injection of turpentine.Results1A group of guinea pigs in a closed abdominal surgery, death. Another block in liver surgery to lift about5minutes after death.2B group was injected with0.3ml of turpentine in guinea pigs24hours after death. Second only to injection0.1ml never regained consciousness after anesthesia in Guinea pigs, death.3C group in the fixed case, but no anesthesia, a guinea pig at the instant when the percutaneous violent struggle, resulting in the abdominal cavity needle stirred, then stopped breathing and heartbeat. Another anesthesia, ultrasound monitoring difficult, puncture bleeding, had to give up.Conclusion1When I inject turpentine injection in the liver, the guinea pig on the turpentine is too sensitive, and the mortality rate is too high.2The guinea pig is not suitable for percutaneous transhepatic experimental animals.3Guinea pig trauma tolerance is not conducive to the postoperative rehabilitation and experimental observations, I should improve the experimental in the further. Part2:turpentine, Staphylococcus suspension with ethanol injection for intrahepatic rabbit liver in ratsMaterials and methods12local adult rabbits, the quality between the1.90～2.20kg, female, by the Institute of Experimental Animal Center of Dali. Randomly divided into four groups:A group of turpentine group; B group aureus group; C group turpentine, Staphylococcus aureus mixed solution series group; D, control group:ethanol group.Result1A group was the control group:sterile normal saline group, Three rabbits in the liver of the injection site, no significant change.2B group of turpentine group, three rabbits, there are two liver abscess formation of the injection site, one lead to local inflammation;3C group, Staphylococcus aureus group, there are two form of liver inflammation injection site, one partial abscess;4Dgroup turpentine-Staphylococcus aureus suspension line group, three rabbits in all the injection site, formation of two liver abscess;5Twelve rabbits, all alive, or inflammation of the liver abscess, antibiotics can control.Conclusion1Percutaneous rabbits as experimental animals, injection of inflammatory agents in the liver tumor is safe.2Turpentine and Staphylococcus aureus suspension sequential intratumoral injection can make the local liver necrosis, suppuration; is the ideal inflammatory agents.3Intratumoral injection of liver inflammatory agents to treat cancer may become a treatment mode!...