Studies On The Chemical Constituents Of Inula Racemosa Hook.f.

There are about100species of the genus Inula widely found in Europe, Asia andAfrica, mostly in Mediterranean, with more than20species being distributed in China.Many of them have long been used in Chinese folk medicine, most frequently employedfor their peptic, relieving phlegm, detumescence, anti-inflammatory and vermifugeproperties. Recently much attention has been paid to Inula species due to their diversebiological activities, which include anti-inflammation, antitumor and anti-bactericidaleffects.Inula racemosa Hook.f. named “ma nu” in zang language, with other name “zongzhuang mu xiang” which is widely distributed in HuBei, ShanXi, XinJiang, QingHai,XiZang province. The root of the plant can be used to the treatment of chronic gastritis、neurodynia, chest contusion. In addition, the report on the chemical constituents andbioactivities of Inula racemosa Hook.f. is very few.The current study aimed to search for novel bioactive compounds from Inularacemosa Hook.f., and had led to the isolation of twenty-seven compounds, includingtwenty-three sesquiterpenoids, three flavones, one steroid. Their structures were identifiedby extensive spectroscopic methods including IR, ESI-MS,¹H-NMR,¹³C-NMR, DEPTand2D-NMR experiments or by comparing their spectral data with those of published inliteratures. Those compounds were identified as follows:ZMX-1racemosin AZMX-2（7R,8R,10R）-8-hydroxy-eudesma-4（5）,11（13）-dien-12-oic acidZMX-3（4S,8R,10R）-13-dimethoxy-eudesma-5（6）,7（11）-dien-12,8-olideZMX-4（4S,8S,10R）-12-hydroxy-eudesma-5（6）,7（11）-dien-12,8-olideZMX-513-hydroxy-8（αH）-eudesma-5,7（11）-dien-8,12-olideZMX-64α-H-eudesm-11（13）-en-4,12-diolZMX-73-oxo-eudesma-4,11-dien-12,8β-olideZMX-811α,13-dihydroalantolactoneZMX-9alantolactoneZMX-10isoalantodieneZMX-11isoalantolactoneZMX-12（1（10）E）-5β-hydroxygermacra-1（10）,4（15）,11-trien-12,8-olide ZMX-132α-hydroxyeudesma-4,11（13）-dien-12,8β-olideZMX-14dehydroivangustinZMX-1511,13-dihydroisolantolactoneZMX-164β,5α-epoxy-1（10）,11（13）-germacradiene-8,12-olideZMX-1713-acetyloxy-5,7（11）-eudesmadien-12,8-olideZMX-184β-hydroxyeudesma-5,11-dien-12,8β-olidZMX-19septuplinolideZMX-20racemosin BZMX-21isoalloalantolactoneZMX-2212-carboxyeudesma-3,11（13）-dieneZMX-232-naphthaleneacetic acidZMX-24velutinZMX-255,14-dihydroxy-7-methoxyflavononeZMX-265,7,4’-trihydroxyflavoneZMX-27β-sitosterolAmong them, ZMX-1, ZMX-2, ZMX-3, ZMX-4were new compounds. CompoundsZMX-7, ZMX-13, ZMX-18, ZMX-19, ZMX-20, ZMX-23were isolated from this plant forthe first time. All the sesquiterpenes were evaluated the inhibitory activities on theLPS-induced NO production in RAW264.7macrophages. Among them, ZMX-9, ZMX-12,ZMX-13exhibited potent inhibitory effects with IC50values followed as7.39±0.36,6.35±0.26,5.39±0.18μM.