| Chronic fatigue syndrome (CFS) is a specific clinical condition that characterisesunexplained disabling fatigue and a combination of non-specific accompanying symptomsfor at least6months, in the absence of a medical diagnosis that would otherwise explainthe clinical presentation. Other common symptoms include headaches, myalgia, arthralgia,and post-exertional malaise; cognitive difficulties, with impaired memory andconcentration; unrefreshing sleep; and mood changes. The estimated worldwide prevalenceof CFS is0.4-1%and it affects over800,000people in the United States andapproximately240,000patients in the UK. No physical examination signs are specific toCFS and no diagnostic tests identify this syndrome. The pathophysiological mechanism ofCFS is unclear. The main hypotheses include altered central nervous system functioningresulting from an abnormal immune response against a common antigen; a neuroendocrinedisturbance; cognitive impairment caused by response to infection or other stimuli insentient people.Hyperbaric oxygen(HBO) treating can improve mitochondrial energy metabolism oncellular level,stabilize intracellular and extracellular electrolyte environment, enhanced therate of lactate removal from peripheral blood vessels.HBO can relieve drug-inducedHIV-associated neuropathy and high-intensity, short-duration exercise performance.1.Objective:1.1Establish an animal model suffered from (Chronic Fatigue Syndrome, CFS), andobserve the effects of hyperbaric oxygen therapy on CFS.1.2Study the effects of hyperbaric oxygen on thyroid stimulating hormone (TSH),tetraiodothyronine (T4) and triiodothyronine (T3), in the CFS rats.1.3study the effects of hyperbaric oxygen on the vascular endothelial function，whichincludes the change of endothelin-1(ET-1),nitric oxide (NO),von willebrandfactor (vWF).1.4study the effects of hyperbaric oxygen on follicle stimulating hormone (FSH),luteinizing hormone(LH) in the CFS rats.2.Methods:2.1SD rats were randomly divided into a blank control group, a hyperbaric oxygen control group, a CFS model group, a CFS hyperbaric oxygen group and a CFS rest group.CFS models were established by using a weight-loaded exhausting swimming method. Thegeneral conditions, the time period of the exhausting swimming, the climbing time periodand the open field time period of rats were evaluated, and the effects of hyperbaric oxygenon rats in the CFS model group were studied.2.2Levels of TSH, T3and T4were determined by a radioimmunoassay.2.3Levels of ET-1,vWF and NO were determined by a radioimmunoassay.2.4Levels of FSH and LH were determined by a radioimmunoassay.3Results:3.1Compared with the rats in the blank control group, the rats in the CFS modelgroup showed worse general conditions, decreased body weight, shortened time period ofthe exhausting swimming and the climbing time period, increased time period in thecentral sector of the open field, decreased numbers of entering into the central sector, anddecreased rest period and times (total seven indexes). Compared with the rats in the CFSmodel group, the above seven indexes for the rats in the CFS hyperbaric oxygen groupbecame better (P <0.05); however, those indexes for the rats in the CFS hyperbaric oxygengroup were still worse than those in the blank control group (P <0.05). Compared withthose in the CFS model group, the seven indexes in the CFS rest group are not significantlyimproved (P <0.05).3.2Compared to the blank control group, the CFS model group has an obviouslydecreased level of T3(P<0.05). Compared to the CFS model group, the CFS group treatedwith the hyperbaric oxygen has a recovered level of T3(P<0.05), which has a statisticalsignificance. Compared to the blank control group and the control group treated with thehyperbaric oxygen, the CFS group treated with the hyperbaric oxygen has a lower level ofT3(P<0.05). There is no significant difference for the levels of TSH and T4in all fivegroups (P>0.05).3.3Compared to the blank control group, the CFS model group has an obviouslyincreased level of ET-1,vWF (P<0.05),and has a decreased level of NO(P<0.05).Compared to the CFS model group, the CFS group treated with the hyperbaricoxygen has a obviously recovered level of ET-1,vWF and NO(P<0.05), which has a statistical significance. Compared to the blank control group and the control group treatedwith the hyperbaric oxygen, the CFS group treated with the hyperbaric oxygen has a samelevel of ET-1,vWF and NO (P>0.05).3.4Compared to the CFS model group and the CFS rest group, CFS group treatedwith the hyperbaric oxygen has an obviously recovered level of FSH and LH (P<0.05),which has a statistical significance. There is no significant difference for the levels of FSHand LH in the blank control group, the CFS group treated with the hyperbaric oxygen,and the control group treated with the hyperbaric oxygen (P>0.05).4.ConclusionCFS can not be released only by rest in rats, and the hyperbaric oxygen therapy canrelease the symptom of CFS. The hyperbaric oxygen hyperbaric oxygen therapy playspositive and active roles in the CSF,and has not obvious effects on the normal rats.Chronic Fatigue Syndrome, hyperbaric oxygen, disease model,，thyroid stimulatinghormone (TSH); tetraiodothyronine (T4); triiodothyronine (T3)；endothelin-1(ET-1);nitricoxide (NO);von willebrandfactor (vWF)，Follicle Stimulating Hormone，（FSH）.;Luteinizing Hormone（LH） and therapy... |
PDF Full Text Request