Experimental Study On Ultrafine Zhikang Yin For Nonalcoholic Fatty Liver Disease

Objective:To observe the effects of Ultrafine Zhikang yin on experimental rat model with Nonalcoholic Fatty Liver Disease/Non-alcoholic steatohepatitis,in terms of histomorphology,oxidative stress and lipid peroxidation,preliminarily to investigate the possible pathogenesis of them.Methods:Sixty-eight SD rats were randomized into two groups at the end of1s week after feeding with normal fodder:fifty-six rats in model group and twelve in blank group(half male and half female).Model group were feed with High Fat Diet and blank group with normal fodder. Six rats in model group and two in blank group(half male and half female)were randomly selected and killed at the end of6th week,the liver tissues were taken to test the pathological morphology.The result suggested that the model was reproduced successfully.Fifty rats (half male and half female) in model were randomly divided into five groups(ten rats one group):model group,Ultrafine Zhikang yin low-dose group,Ultrafine Zhikang yin middle-dose group,Ultrafine Zhikang yin high-dose group and Diammonium Glycyrrhizinate Enteric-coated Capsules(Gancaosuan Er’an Changrong Jiaonang)group.Then the blank group was feed with normal fodder, the other groups were feed with high fat diet and treated by Ultrafine Zhikang yin in different doses at the same time.All rats were killed and taken the liver tissues at the end of6th week after treatment.Then observed the histopathology and histomorphology of the rat’s liver,detected the content of FFA,SOD, MDA of liver homogenate by Elisa and the expression of COX-2in rat’s liver by Immunohistochemistry and Western Blot.Results:1.The structure failure of hepatic lobule and hepatic sinus,arrange in disorder of hepatic cord, moderate to severe fatty degeneration, more ballooning degeneration of rat’s liver in model group were displayed at light microscopic level,but,obvious inflammatoryinfiltration was not been found.Compared with the model group,mild to moderate fatty degeneration and little ballooning degeneration were found in Ultrafine Zhikang yin groups,inflammatoryinfiltration and spotty necrosis also not been found in hepatic lobule.2.NAS integral of model group was more than the blank one,there was a significant difference which had statistical significance(P<0.05);NAS integral of all treatment groups was less than model(P<0.05);There was no statistical significance between Gancaosuan Er’an Changrong Jiaonang group and Ultrafine Zhikang yin groups(P>0.05).3.The content of FFA、MDA、COX-2in liver homogenate could be reduced and SOD increased by Ultrafine Zhikang yin(P<0.05);Compared with Gancaosuan Er’an Changrong Jiaonang group, there was no obvious difference between them,and the efficacy were similar(P>0.05).4.The results of light microscopic and western blot demonstrated that Ultrafine Zhikang yin groups could reduced the express of COX-2in liver tissue (P<0.05);Compared with Gancaosuan Er’an Changrong Jiaonang group,there was no significant difference(P>0.05).Conclusions:1.This experiment have developed a rat NASH model by using improved high fat diet, but a sole animal model,which is contain the whole spectrum of disease of NAFLD,were not been found. The pathologic results of model rat’s liver showed moderate to severe fatty degeneration,ballooning degeneration was common,but the inflammation activities were not obvious.2. The pathologic result of this experiment testified that Ultrafine Zhikang yin had obvious treatment effect,and the effect of middle group was better.3. The mechanism of action of prevention and curative in NAFLD/NASH by Ultrafine Zhikang yin maybe protecting the liver cell by reducing the fat content and relieving its lipotoxicity,relieving oxidative stress by improving the oxidation resistance of liver cell,avoiding the damage of liver by reducing LPO and its metabolin.