| Research of tripdiolide and its analogues on diabetic nephropathy(DN)biochemical values in blood and urine of rats effects, on renal tissue and serumtransforming growth factorβ1(TGF-β1) effect and function of content, on vascularendothelial growth factor (VEGF) in the kidney expression of, on renal tissue repair andthe treatment of DN.Healthy male and female SD rats and60, by weight, randomly divided into5groups:blank control group A, experimental control group B, a combination of tripdiolide andtriptolide treatment group C, a combination of tripdiolide and valsartan treatment groupD,valsartan treatment group E. Each set of12rats, with four groups of B, C, D, E, effectsof intraperitoneal injection of chain combined (STZ) build DN rat models. One week afterthe model of success, c, d, e are intragastric administration, and were collected at0,4,8,12weeks24h urine specimens. Treatment after12weeks, one kill rats, collecting bloodand kidney specimen in rats, testing blood glucose(BG), serum creatinine(SCR), bloodurea nitrogen(BUN), total cholesterol(TC), triglycerides(TG) and24-hour urine proteinquantitative as well as quantitative values of body fluid protein. Anatomy of rat, leave akidney and liver specimen, weighing. On the set of specimens for routine HE staindetection of pathology of the kidney, see DN repair of kidney structure; detected byimmunohistochemical SABC group positive expression of VEGF in the kidney; enzymelinked immunosorbent assay (ELISA) with resistance to sandwich assay TGF-β1in RatRenal tissue and serum of the actual content;Using high performance liquidchromatography (HPLC) testing treatment groups in the rat kidney, liver and serum contentof tripterygium wilfordii and its analogues in accumulation and metabolism of drugs.By HPLC detection to Tripdiolide and its analogues in large rat kidney and serum inthe of metabolism accumulation, effective reduced reduced has DN large rat24h urinevolume, and urine protein quantitative, and BG, and BUN, and TC, and TG all value,tripterygium wilfordii class drug not only regulation has TGF-β1in kidney organizationwithin and serum in the of content, and effect DN large rat kidney organization in the VEGF and TGF-β1of expression, on DN large rat kidney up to protection and repair ofrole, effective cure DN large rat.Of tripdiolide and its analogues in the DN in the serum of rats and accumulatedstorage, and by reducing the contents of renal TGF-inβ1, inhibition of serum TGF-inβ1onthe expression of VEGF-induced, to repair and protect kidney function as well as on thetreatment to the DN. |
