The Study Of The Effect Of Licorice And Codonopsis Extracts On IEC-6Cell Migration And Polyamines

BackgroundSpleen Deficiency Syndrome is a gastrointestinal disease syndromes, gastrointestinal mucosal protection is one of the main mechanism of the Yiqi Jianpi herbs.Gastrointestinal mucosa protection is main content of traditional Chinese medicine "spleen function", and repair of gastrointestinal mucosal injury is an important material basis of the Yiqi Jianpi herbs in Chinese medicine. Epithelial injury of the gastrointestinal mucosa have the ability to repair itself, generally the first step is cell migration. A important step of mucosal repair is cell migration or not and whether to the injury site in migration from non-injury site. In the process of protection and repair of gastrointestinal mucosa, the polyamine-mediated potassium channel activation signaling pathway plays an important role. Polyamines increase the enhanced K+channel protein expression of K+outflow, leading to hyperpolarization of the membrane, on the contrary, elimination of polyamines inhibits cell migration.Research purposesObserved the effects of Yiqi Jianpi herbs licorice, Codonopsis on IEC-6(intestinal epithelial cells) migration, polyamine content and the effects on cell migration (4-Ap as inhibition durgs). Study gastrointestinal mucosal damage repair mechanisms of licorice and Codonopsis.Research MethodsThe laboratory has been established intestinal epithelial cells (IEC-6) migration pharmacological experimental model. the test drugs and tool drugs "spermidine" to promote the efficacy of cell migration on cell migration were observed to determine whether effective the investigational drug. And screen the effective parts of the test drug. cell migration Inhibited by DFMO (Irreversible specific inhibitor of polyamines) and4-AP (potassium inhibitor), then add the investigational drug to study the mechanism of the test drug to repair of gastrointestinal mucosal injury.Detectec intracellular content of polyamines (spermidine) used Pre-column derivatization high performance liquid chromatography of cell migration,.The results 1. Licorice extract50、100mg/L, the licorice flavonoids of50、100mg/L, and the Codonopsis sugar extract25、50、100mg/L, and Codonopsis flavonoids50、100mg/L, the migration of IEC-6cells promote.licorice extract25mg/L, and licorice flavonoids25mg/L, glycyrrhizin25、50、100mg/L, and Codonopsis saponin25、50、100mg/L, the migration of IEC-6cells and has no effect.2. Licorice extract50、100、200mg/L, licorice flavonoids100、200mg/L, Codonopsis sugar extract50、100、200mg/L, Codonopsis flavonoids50、100、200mg/L promote the migration of IEC-6cells added the inhibitor DFMO.3.12hours after cell administration, the licorice extract50、100mg/L, licorice flavonoids50、100mg/L, Codonopsis sugar extract100mg/L, and Codonopsis flavonoids100mg/L, can significantly improve the content of polyamines,24hours after the cell administration of licorice extract50、100mg/L, and licorice flavonoids100mg/L, can significantly improve the content of polyamines.4. Licorice extract50mg、100、200mg/L, and the Codonopsis sugar extract50、100、200mg/L, the licorice flavonoids50、100、200mg/L, and the party Participation flavonoids50、100、200mg/L of IEC-6cell migration promoted under the load of the potassium channel inhibitor4-AP.ConclusionsDuring Glycyrrhiza and Codonopsis gastrointestinal mucosal injury repair. licoricesugar extract,Codonopsis sugar extract, licorice flavonoids, Codonopsis flavonoids at work, and about promoting the intestinal epithelial cell migration after mucosal injury. the mechanism may promote polyamine generation the promotion of the outflow of potassium after mucosal injury.