Study On The Determination Of Rosmarinic Acid In Chinese Herbal Medicines And Cosmetics By Capillary Electrophoresis

Rosmarinic acid was found from the extracts of rosemary by Ellis in1958. It was used widely in the fields of pharmaceutical, chemical, cosmetics due to its medicinal value and cosmetology effection. Thus, analysis research on rosmarinic acid is of great significance. Because of lower concentration sensitivity of capillary electrophoresis with UV-Visible detection, it is important to propose new methods for the determination of rosmarinic acid in Chinese herbal medicines or cosmetics. Since the application of CE on herbal medicine is already in process, futher investigation should be made to meet the demand for quality control of Rosemary. Besides, there has not seen previous reports on the determination of analytes in cosmetic by CE. Therefore, author chose the subject of dessertation according to the development trends combined with the actual situation of the laboratory in the paper. The main contents of the desertion were as follows:1. Study on extraction of rosemary acid of SalviaExtraction conditions of rosemary acid in Salvia were studied in the paper. Using ultrasonic extraction method, in single factor and level conditions, the orthogonal design condition contain:the ratio of solid liquid, methanol concentration, extraction temperature and extraction time. Qualitative and quantitative analysis of extracted content of rosmarinic acid of Salvia miltiorrhiza was done by method of capillary electrophoresis and used as validation index. Results showed that optimum experimental conditions were:ratio of material liquid1:25,50%methanol, temperature75℃, extraction time30min.2.Determination of rosmarinic acid in salvia miltiorrhiza and compound salvia tablets by micellar electrokinetic capillary electrophoresisA new method based on micellar electrokinetic capillary electrophoresis has been developed for determination of rosmarinic acid in salvia miltiorrhiza and compound salvia miltiorrhiza tablet. Using the inject method of gravity, the sample was injected in75s, the buffer solution was composed of25mmol/L borax-40mmol/L SDS, pH was9.85, the applied voltage was13kV, detection wavelength was set at320nm. Under the optimum conditions, linear equation of RA was y=18117x+3157.4, the correlation was0.9968. The linear range was2.5-100μg/mL and the detection limits was0.25μg/mL. The recovery found were in the range from92%to105%, with RSD of3.2%.The method is simple and accurate. It can be used to detect rosmarinic acid in salvia miltiorrhiza and compound salvia tablets.3.Determination of rosmarinic acid in cosmetics by Dynamic pH Junction-sweeping in capillary electrophoresisA method of dynamic pH junction-sweeping in capillary electrophoresis was achieved for the analysis of rosmarinic acid in cosmetics. In the case of the sample height is15cm by gravity sampling, the study of some conditions which are the concentration of borax, pH value, the concentration of SDS, the concentration of methanol, sample matrix, sampling time, separation voltage were optimized. The optimized experimental condition:the buffer:15mmol/Lborax-45mmol/L SDS(pH8.8)-15%methanol, the sampling time was60s, the applied voltage was16kV, the concentration of phosphate in the sample was10mmol/L. The pH of sample matrix was4.7.The linear equation of RA was y=539200x+53588, the correlation was0.9985.The linear range was0.144-3.6μg/mL and the detection limits was0.036μg/mL. The recovery found was in the range from92.5%to103%, with RSD of2.5%.4.Study on Capillary Electrophoresis specific chromatogram of rosmarinus officinalisThe electrophoretic specific chromatogram of water-soluble constituents of rsomarinus officinalis was established by capillary electrophoresis.20mmol/L borax (pH=9.50) was used as background electrolyte, and running voltage of12kV was applied. UV-detection at the wavelength of300nm was adopted. Samples of rosmarinus officinalis in10different areas were taken for analysis. Base on the value of rate of common elect rophoretic peaks fi≥90%, a set of electrophoretic characteristic atlas of11peaks was determined for flavones in rsomarinus officinalis samples from10different areas. Good similarities between the electrophoretic finger print atlases of the samples and the standards were obtained. Relative standard deviation among the values of relative migration times of various specific peaks obtained at different time of sample introduction was less than5.0%, and values of RSD for the relative peak areas were in the range from2.7%to8.0%. The results obtained proved that the sample was stable within36h. The method is simple, accurate and suitable for the quality control of rosmarinus officinalis.