Study On The Expression And Correlation Of EGFR. TTF-1、Ck7、Ki-67and P53in Non-small Cell Lung Carcinoma

The Non-small-cell lung carcinoma is a common type of primarypulmonary carcinoma. The most common pathological types contain squamouscell carcinoma, adenocarcinoma and magnocellular carcinoma, occasionally,you can also see some other types of a low incidence or a mixed cell type. Thepathological classification was based primarily on evaluating the histologicaltypes, while immunohistochemical stains could be used to identify primarylung adenocarcinoma and metastatic adenocarcinoma of the lung adenocar-cinoma, adenocarcinoma and malignant pleural mesothelioma, and todetermine the tumor’s nerveclosely related to the endocrine status. Themolecular level anomalies of lung carcinoma may be used to predict sensitivityand resistance of the epidermal growth factor receptor tyrosine kinase inhibitors(EGFR-TKI). According to the pathological assessment principles releasedby2011National Comprehensive Cancer Network (NCCN): the mutation rateof epidermal growth factor receptor (EGFR) in Adenocarcinoma is10%in thewestern countries, and50%in Asian population. And it has higher mutationrate in non-smokers, women and the mucous cancer patients. Thyroidtranscription factors-1(TTF-1) is essential for the identification of primary andmetastatic adenocarcinoma, and the majority of primary lung adenocarcinomaTTF-1is positive expression, yet metastatic lung adenocarcinoma almost isnegative expression; Usually in the primary lung adenocarcinoma, keratincells-7(CK7) is positive expression, cell keratin-20(CK20) is negativeexpression, and the colorectal adenocarcinoma lung metastases performance forCK7-/CK20+. China’s Ministry of Health in2011of lung cancer diagnosis andtreatment guidelines on lung cancer pathology report shows that immuno-histochemistry for the differential diagnosis of lung cancer include: squamous cell carcinoma of the key screening CK14, CK5/6,34βE12and p63; lungadenocarcinoma focus on screening for CK7and TTF-l; lung neuroendocrinecarcinoma of the key screening CK18, AE1/AE3, CD56, CgA and NSE, andSyn.In recent years, research on Ki-67，which is as the most extensiveproliferation cells markers has made good progress，as is on lung cancerhistologic type, differentiation degree and prognosis relationship. Research onCorrelation between Gene mutations together with protein expression on P53which is as the most important tumor-suppressor genes, and biological actionsand clinicopathologic features of lung cancer, though there is a lot ofcontroversy, has made important progress. Therefore, this experiment selectedfive indicators, such as EGFR, TTF-1, CK7, Ki-67and P53, detected the aboveindexes expression in351NSCLC paraffin section by immunohistochemicalmethod (IHC), and did the correlation analysis on the relationship between theexpression of all the indexes and NSCLC pathological features by evidence-based medicine method.The results show that positive expression rate of EGFR、TTF-1、CK7、Ki-67and P53in351lung cancer groups were respectively60.68%,43.87%,43.87%,84.05%and60.11%. EGFR was closely related with thehistory of smoking of patients with lung cancer (P<0.05).TTF-1was closelyrelated with sex of the patients, the history of smoking, lung cancerpathological types and pTNM (P<0.05). CK7was closely related with thehistory of smoking, sex and lung cancer pathological types (P<0.05). Ki-67wasclosely related with the history of smoking, sex and lung cancer pathologicaltypes (P<0.05). P53was closely related with lung cancer staging made byWHO (P<0.05). Correlation exists in expressions of combination betweenTTF-1&CK7in lung cancer group，scale cancer group and adenocarcinomagroup, and the difference was statistically significant.