The Research Of Helicobacter Pylori Toxins Related Proteins CagA Promote The Epithelial-mesenchymal Transition Of Gastric Epithelium Cells Through MicroRNA

Objectives To explore the pathogenic mechanism in the development of Epithelial-mesenchymal transition(EMT) in gastric epithelium cells of the helicobacter pylori(Hp) toxins related proteins CagA.Methods We established the AGS cells which is stable expressed of CagA first, Then used mammals miRNA expression chip to test the CagA transformation of AGS control stomach cancer cells and empty carrier cells to screen potential changes microRNA, Used fluorescence insect enzyme gene report method and western blot to test the corresponding miRNA common role of molecular targets, Through knockdown the molecular targets to observe the influence of the EMT of the stomach epithelial cells and the proportion of stem cells.Results We found that the hsa-mir-584and1290expressed appears raised in the transformation of CagA cells, Pioneer transcription factors FoxAl is the common role of molecular target of has-mir-584and1290, The percentage of CD44+and CD133+ cells appear significantly less in the SW620cells with FoxAl knockdown, However, the percentage of CD24+cells was significantly increased, P<0.05. The cell morphology with FoxAl knockdown become from the spindle into a small round. The E-cadherin expression was decreased by western blot detected,while the Vimintin expression was increased.Conclusion hsa-mir-584and-1290promote the EMT process and interfere with cell development and differentiation by down FoxAl. Through the miRNA pathway is a new pathogenic mechanism of CagA protein.