Expeirmental Study Of The Functions Of GDNF And DbcAMP On The Induction Of Differentiation Of Neural Stem Cells Into Dopaminergic Neurons

Neural stem cells are a class of self-renewing, pluripotent parent cells.they are able to differentiate into neurons, astrocytes and fewer oligoden-drocytes in certain circumstances. It is precisely because of these characteristics,neural stem cells become the ideal choice for cell replacement therapy andbring new hope for treatment for nervous system damage and degenerativediseases (such as Parkinson’s disease).This test can obtain a considerablenumber of dopaminergic cells through the use of GDNF and dbcAMP on ratembryonic neural stem cells for directed differentiation and therefore lay thefoundation for the next cell transplantation.Method: Isolated and cultured14-16days pregnant Wistar rat neural stem cells, subculture to detect its self-renewalcapacity and its Nestin and Brdu protein, identification, and then take use ofGDNF and dbcAMP directed differentiation of rat neural stem cells, theexpression of TH positive cells detected by immunoc-ytochemistry andimmunofluorescence methods.Result: Cultured neural stem cells express Nestinand Brdu.A significant increase in the number of neurospheres after one weekof in vitro culture as well as the volume increases. GDNF and can increase THexpression respectively and there are significant differences compared with theserum control group（p<0.05）. Conclusion: In the role of the neurotrophicfactor, neural stem cells can rapidly proliferate and form neurospheres.BothGDNF and dbcAMP can result in the induction of differentiation of neural stemcells into dopaminergic neurons, GDNF induces the highest performance in20ug/l and dbcAMP induces the highest performance in200umol/l,both in adoes-dependent.There is significant difference in TH cells positive rate betweencombination of induction and independently induction.