Studies On The Extraction And Content Determination Of Shenguibaoganshengbai Granules

The Shenguibaoganshengbai granules is compound Chinese medicine preparationswhich is composed of thirteen kinds of TCM,ginseng, licorice, figwort, red peony and so on.It has the effect of activating blood circulation to dissipate blood stasis and invigorating vitalenergy.The former pharmacological research also proved that SGG has effect of protectingliver, promoting albumin and reversing the cirrhosis forming according to a famous Chinesephysician with high reputation in Jilin Province by many years of clinical experience.SGG belongs to the Sixth Type of New Traditional Chinese Medicine. We establish itspreparation process and quality control standards research according to the procedures forexamination and approval of new drugs, and choose particle form as the agents and developfurther study on the extraction and content determination of Shenguibaoganshengbaigranules.The results are as follows:1.research of the extraction process（1） alcohol extraction processRed peony root, Scrophulariaceae, medicinal herbs D mixture of three kinds of Chineseherbal medicines with different concentrations of ethanol reflux extraction as an index to thecontent of harpagoside from Scrophularia. Research the ethanol concentration, extractiontime, extraction time impact on Harpagoside content. Derived the Red peony root,Scrophulariaceae, medicinal herbs D extract the optimum conditions which are3h,2h,2h,theamount of ethanol as6mL/g、4mL/g、4mL/g.（2） boiling water extraction processThe main chemical ingredient of Chinese medicinal materials soluble in water were acombination of boiling water, ginseng water extract of ginseng, ginsenoside Rg1, Re, andginsenoside Rb1assessment indicators; Water extract containing licorice and other herbscombination of glycyrrhizic acid in licorice for the assessment indicators, the optimumconditions investigated boiling water: Take the number3, the extraction time is followed by 3h,2h,2h, add water to6mL/g,4mL/g,4mL/g.（3） the process of steam distillationSteam distillation of the ingredients A, medicinal herbs B and medicinal herbs C mixedin water and soak for2h, and then collect the distillate to deal with the volatile oil. Vol umeof volatile oil derived from the index, the extraction time to inspect to determine the time ofsteam distillation for18h. The residue collected boiling water for2times,2h, plus water4mL/g.2. study on determination of contentThe qualitative TLC is well researched in the dissertation. The medicine of Red peony isone specific component. We can conclude that the blue spots on the chromatograms are clearand characteristic, without interference by negative material from The experimental results.Three batches of samples have good reproducibility. So the methods are very suitable for thequalitative identification of SGG.Using HPLC to test the content of Glycyrrhizic acid from licorice and Harpagoside fromScrophulariaceae.When determinate the content of Glycyrrhizic acid, Chromatographicexperiments were performed on column Phenomenex column(250mm*4.6mm,5μm),themobile phase wasacetonitrile-water with0.4%phosphoric acid (34:66);the detectionwavelength was254nm, the flow rate was1.0mL/min. The linear range for detection ofGlycyrrhizic acid was0.46μg～4.14μg/mL (r=0.9999). The average recovery rate was95.9%,with RSD=2.1%(n=6).The content of Glycyrrhizic acid is no less than115μg pergranule. The method is accurate, stable so we can use it as determination control of thepreparation. When determinate the content of Harpagoside, Chromatographic experimentswere performed on column Phenomenex column(250mm*4.6mm,5μm), acetonitrile is themobile phase A, water with1%phosphoric acid is the mobile phase B;0-20minutes,A:12�'40,B：88�'60;the detection wavelength was278nm, the flow rate was1.0mL/min. The linear range for detection of Harpagoside was0.13μg～1.05μg/mL (r=0.9999).The average recovery rate was96.4%with RSD=3.5%(n=6).The content of Harpagoside isno less than60μg per granule. The method is accurate, stable so we can also use it asdetermination quality control of the preparation. When determinate the content ofGinsenoside Rg1, Chromatographic experiments were performed on column AlltimaTMC18 (250mm*4.6mm,5μm),the mobile phase wasacetonitrile–water with0.05%phosphoricacid (21:79);the detection wavelength was203nm, the flow rate was1.0mL/min. The linearrange for detection of Ginsenoside Rg1was1.0μg～8.0μg/mL(r=0.9999).The averagerecovery rate was96.3%,with RSD=2.6%(n=6).The content of Ginsenoside Rg1is no lessthan160μg per granule. The method is accurate, stable so we can also use it as determinationquality control of the preparation.