Schisandra Chinensis Polysaccharide Induce Apoptosis Of Tumor Cell And Its Molecular Mechanism

Tumors,especially malignant tumors has become an important disease of aserious threat to human health. With the in-depth exploration of chinese traditionalmedicine for cancer treatment,the important value of traditional Chinese medicine intumor therapy is also affirmed by some doctors.The tumor is a cell cycle disease.Ascell malignant proliferation and differentiation blocked,abnormal apoptosis plays animportant role in the pathogenesis of the most malignant tumors.The effect andmachanisms of chinese herbs on tumor cell proliferation and apoptosis blocked hasbecome one of the hotspots for research on tumor.Objective: The objective of this project was to study the anti-tumor effect of Chinesemedicine schisandra chinensis polysaccharide (SCP) in vitro, and explore themolecular mechanism of SCP-induced tumor cell apoptosis. This experiment was toprovide theoretical basis for the clinical application of the schisandra chinensispolysaccharide anti-tumor.Methods:The effect of SCP on the growth inhibition of tumor cells in vitro wasdetected by MTT; SCP induced Hela cell apoptosis by Hoechst33342staining wereobserved; The expression of tumor cell apoptosis-related factors P53、Caspase-3、Bcl-2and Bax apoptosis gene were examined by RT-PCR;The expression ofCaspase-3、Bax、Bcl-2protein of tumor cell were detected by western-blot. Theexpression of Active-caspase-3protein by Hoechst33342and texas red doublestaining were detected.Results:1. Tumor cells were treated with SCP for24h and48h, the results of MTT showed thatSCP could inhibit proliferation of tumor cells Hela、MCF-7、SMMC7721、RAW264.7,in2.5、5、10mg/ml. They were significantly inhibited (p <0.05or p <0.01); Hela cells which was treated with SCP showed a dose-dependent inhibition; When SCP treatednormal mouse spleen cells for48h, it had no significant effect on proliferative activityof normal mouse spleen cells.2. Hela cells were treated with SCP for48h, the group of SCP treated showed obviouscharacteristics of apoptosis by hoechst33342staining, such as the increase inmembrane permeability and nuclei were chunky dense stain, while the control groupdidn’t show apotosis characteristics.3. Hela cells were treated with SCP for48h, we detected the expression of Bax、Bcl-2、p53and Caspase-3mRNA by RT-PCR.the results showed that compared to thecontrol group, the group of cells treated with5mg/ml SCP, the Bcl-2mRNAexpression was significantly reduced, its Bax、p53and Caspase-3mRNA expressionwere significantly increased; the group cells treated by2.5mg/ml SCP, and itsCaspase-3mRNA expression had a slight increase, Bax、p53and Bcl-2mRNA had nosignificant effects.4. Hela cells were treated with SCP for48h, we detected the expression of Bax、Bcl-2and Caspase-3protein by Western-blotting. The results showed that compared tonormal control cells,the group cells treated with SCP (2.5mg/ml、5mg/ml、10mg/ml),its expression of Bcl-2protein decreased significantly, its expression of Bax andCaspase-3protein was significantly increased.5. Hela cells were treated with SCP for48h,In confocal microscope, we detected theexpression of Active Caspase-3protein by Hoechst33342and Texas red staining.Compared to normal control cells, the results showed that the expression of ActiveCaspase-3protein of the group treated with SCP (2.5mg/ml、5mg/ml、10mg/ml)significantly increased, and were in a dose dependent relationship.Conclusion:1. SCP could inhibit a variety of tumor cell proliferative activity, and had the effectsof anti-tumor.2. SCP could exert antitumor effects by promoting tumor cell apoptosis.3. SCP could regulate the tumor cell apoptosis factor gene and protein expression ofthe molecular mechanisms mediated anti-tumor effect. 4. In this study, a theoretical foundation and experimental basis for the clinicalapplication of Chinese medicine SCP treatment of cancer.