Association Analysis Of The Serum Level Of Clusterin And Clusterin Gene Polymorphism In Alzheimer’s Disease

Objective:The purpose of the study was to survey the relationship between clusterin ge ne polymorphism and AD in Chinese; to measure the serum clusterin of level,and study the level and significance of the clusterin in Alzheimer’s disease (AD)Methods:1.Collect AD and healthy normal controls cases.2.Two groups of patients were take3ml peripheral venous blood, take serum.3.By using hypotonic hemolysis, conventional phenol-chloroform extraction of white cel1genomic DNA.4.Polymerase chains re-action restrictions fragment length technology examined with the genotypes and allelic frequencies of clusterin gene5.The serum clusterin of level patients with AD(observed group) and healthy normal cont rols (control group) were measured by enzyme-linked immunosorbent assay(ELISA).Results:1. The C/C genotype and C allelic frequencies of clusterin gene was more general in the Alzheimer’s disease group than in the normal group, the distribution of C/C genotype a nd C allelic frequencies was different between AD and normals(X2=6.673, P＜0.05)(X2=15.060, P＜0.05);an association of C allelic and C/C genotype with AD (OR=3.685,95%C1=1.855-7.320)(OR=2.750,95%Cl=1.260-6.002) was observed;2.The serum clusterin level of the observed group was higher than the normal group (P＜0.01).Conclusion:The C/C genotype and allelic C of clusterin gene was associated with AD.; The serum clusterin level of patients with AD was significantly higher than the control g roup,the result with the foreign coverage is basically the same.The serum clusterin may play an important role in the occurrence and development of AD.