The Expression Of Rhoc In Human Glioma And Its Clinical Significance

Objective To investigate the expression of RhoC in human glioma and the correlation between RhoC’s expression and pathological grade, futhermore, whose clinical significance would be discussed for diagnosing the glioma, judging the malignant grades and the patients’prognosis.Methods The expression of RhoC protein and RhoC mRNA was detected in63cases of human glioma samples and11cases of normal brain tissues by SABC immunohistochemical staining and reverse transcription polymerase chain reaction(RT-PCR), respectively.Results①The immunohistochemistry showed that the ratio of RhoC positive cells was100%in all detected62human glioma samples while there was no expression in11normal brain tissue samples.②The ratio of RhoC positive cells and the gray degree ratios of the RhoC mRNA belt relative to the GAPDH mRNA belt varied accordingly to different pathologic grades. There were differences between grade I or grade II and grade III or grade IV(P<0.05), and the expressions of RhoC mRNA in grade III or grade IV were significantly more than those of grade I or grade II.③Analysis of correlation:The positive correlation existed between the ratio of RhoC positive cells or RhoC mRNA and the pathologic grading of the human glioma (r=0.814,r=0.863,P＜0.001)Conclusion RhoC was present in all62cases glioma samples, whose expression was grade correlation positively, which suggestted that RhoC may serve as novel and efficient diagnostic markers for astrocytic brain tumors of histological grade I to IV and complement currently applied histopathological analysis.