The Expression Of CXCL12 And CXCR4 In Human Glioma And Its Significance

Background and ObjectiveGlioma is the common tumors in central nervous system, which accounts for approximately 35.12%～61.10% of intracranial tumor. Most of them shows infiltrative growth, and it has such characteristics as high mothidity, high relapse rate, high mortality and low cure rate. The pathogenesis is unclear, though the comprehensive treatment methods that surgical treatment combined with radiotherapy、chemotherapy and immunotherapy has achieved a certain clinical effect, the overall prognosis of patients with glioma has not fundamentally improved. Therefore, to clarify the pathogenesis of glioma and to search for the effective treatment measures associated with the pathogenesis have become important issues for the current study.Modern molcular biology considers that the occurrence and development of tumor are multi-gene、multi-factor、multi-step and multi-stage synergistic complex processes. Chemokines are a class of chemical inducer of cytokines that can promote inflammation, and it can cause chemotaxis、cell survival and/or cell proliferation、increase of intracellular calcium、gene transcription and so on. With the development of research, people have found that chemokine CXCL12 (CXC motif ligand 12) and its receptor CXCR4 (CXC motif receptor 4) was closely related to the occurrence and development of some tumors. The combination of CXCL12 and CXCR4 may be involved in the occurence、proliferation and/or survival、angiogenesis、metastasis、immunosuppression and other processes of tumor through the downstream of multiple signaling pathways, now the CXCL12 and CXCR4 has become one of the hot spots in the related field of tumor molecular biology. For the further study of the expression of CXCL12 and CXCR4 in human glioma and its significance, RT-PCR technique was used to detect the expression of CXCL12mRNA and CXCR4mRNA in non-tumor brain tissues and glioma in order to explore the roles of them in the occurrence and development of glioma, aimed at providing new ideas for the clinical treatment of glioma.Materials and Methods48 cases of fresh human glioma specimens which were surgically removed and confirmed by pathology were collected in the Department of Neurosurgery of The First and The Second Affilitated Hospital of Zhengzhou University from March 2010 to July 2011, including 31 cases of male and 17 csaes of female and all cases were primary cases. According to the classification and grading of central nervous tumors of WHO (2007),8 cases of grade I,17 cases of gradeⅡ,16 cases of gradeⅢ, and 7 cases of grade IV.25 cases of gradesⅠ-Ⅱwere classificated as low-grade group and 23 cases of gradesⅢ-Ⅳwere classificated as high-grade group. The non-tumor brain tissues were taken from 12 patients implemented surgical decompression due to traumatic brain injury. In this study, RT-PCR technique was used to detect the expression of CXCL12 mRNA and CXCR4 mRNA in non-tumor brain tissues and glioma. The data were analysed by SPSS 17.0 software package, significance levelα=0.05, a value less than 0.05 was considered as significance.Results1. The expression of CXCL12mRNAThe RIOD of CXCL12mRNA in non-tumor brain tissues、glioma、low-grade group、and high-grade group was 0.381±0.19、0.799±0.219、0.680±0.201、and 0.930±0.158. There were significantly differences between non-tumor brain tissues and glioma, low-grade group and high-grade group(P<0.05) 2. The expression of CXCR4 mRNAThe RIOD of CXCR4 mRNA in non-tumor brain tissues、glioma、low-grade group、and high-grade group was 0.226±0.117、0.677±0.231、0.516±0.183、and 0.853±0.124. There were significantly differences between non-tumor brain tissues and glioma, low-grade group and high-grade group (P<0.05)3. The correlation of the expression of CXCL12mRNA and CXCL 4mRNAThe RIOD of CXCL12mRNA and CXCL4mRNA in glioma were 0.799±0.219 and 0.677±0.231, and the two sets of data were all in line with normal distribution by the K-S test, the Pearson product moment correlation coefficient r=0.697, it was satistically significant (P<0.05)Conclusions1. The expression of CXCL 12 and CXCR4 in glioma is higher than that in non-tumor brain tissues, and the expression level of them increases with the upgrade of pathological grade of glioma, the high expression of CXCL12 and CXCR4 may play important roles in the occurrence and development of glioma.2. The expression of CXCL12 and CXCR4 in glioma shows a significant positive correlation, it accords with the ligand-receptor binding characteristics.Detecting the expression of CXCL12 and CXCR4 is expected to provide new ideas for the clinical treatment of glioma.