| BackgroundEsophageal cancer is a common malignant tumor, the new cases per year is approximately400thousand cases all of the world, the incidence of this disease is quite different in different areas, developing countries have the higher incidence. China is one of the higher incidence areas of esophageal cancer, the area mainly distributed around the Taihang mountain area, and Sichuan province. The pathology of esophageal cancer is Squamous cell carcinoma, accounts about95%, other types of the disease is low. Esophageal in different places morbidity and mortality have obvious difference, China, as the high for about each year about150thousand patients died of esophageal carcinomas. Epidemiological studies have shown that, the occurrence of esophageal cancer is closely associated with genetic factors, dietary habits, living environment, race, occupation, age, gender, geographical environment, etc. With the development of molecular biology, found that the occurrence of esophageal cancer development is regulated by a variety of aberrant gene affects the expression, is one of many factors the interaction term results.Pinl is a relative mass of18kD peptide prolyl acyl cis trans isomerase, it can change in the proline peptide bond formation, and only phosphorylated pSer/Thr-Pro isomerization, this institutionthe occurrence of the type of change in the coming dephosphorylation change the catalytic activity, change the protein stability and activity affect the protein between changing the normal subcellular localization, plays an important role in causing cell proliferation and tumor. The latest studies have shown that this regulatory mechanism plays an important regulatory role in a variety of physiological processes, once the disorder will lead to a range of human diseases. mTOR is the rapamycin target protein is a serine/threonine kinase, plays a very important role in the nutritional signals regulate cell growth and cell proliferation. The mTOR have a important affect in the process of cell growth, mTOR is also involved in protein translation initiation, gene transcription, apoptosis and other biological processes, the abnormal expression of mTOR signaling proteins go the same way and the occurrence of malignant cells closely linked, is an important target in cancer therapy.Domestic studies have not been reported the correlation expression of Pinl and mTOR in esophageal carcinoma, the relationship of esophageal cancer progression and invasion and metastasisthe is not very clear. Therefore, detect the esophageal carcinoma cells in Pinl and the mTOR protein, to explore the two mechanism in the formation of esophageal cancer as well as the relationship between the clinical and pathological features of esophageal cancer, analysis of the correlation between the two, to further provide a theoretical basis for the diagnosis and clinical treatment of esophageal cancer.Materials and Methods1. PatientsCollected from July2010to the May2011Zhengzhou University Second Affiliated Hospital thoracic surgery resection of esophageal squamous cell carcinoma (ESCC) specimens and adjacent normal tissue or normal esophageal tissue (NET) specimens, including40cases esophageal squamous cell carcinoma,40cases normal controls. All cases were carried out in accordance with the standards of the International Union Against Cancer UICC pathological staging. All cases have not preoperative radiotherapy and chemotherapy.2. MethodsEach specimen was fixed by10%formaldehyde solution, embedded in paraffin,4mm thick serial slices, using SP immunohistochemical staining,immunohistochemistry operation according to reagent box instructions. Citric acid buffer solution with microwave antigen retrieval, PBS instead of as a negative control.3.The results of immunohistochemistryPinl protein expression are mainly located in tumor cell nuclei, cytoplasm a small amount of coloring, the nucleus or cytoplasm was brown positive; of mTOR protein expression is mainly located in the cytoplasm of tumor cells, the yellow particle-like substances. Specimens randomly selected10high-power field, counting100cells per field of vision, according to the percentage of positive cells and the color depth results to determine, calculate the percentage of positive cells to determine, positive cells of Pinl and mTOR staining results classification of<25%to0,25%to50%for1min,51%to75%for2points,>75%for3minutes; staining intensity scoring staining score was0, yellow1yellow2yellow3. Finally, multiplying the two scores>3is divided into positive expression.4Statistical methodsThe data were analyzed by SPSS17.0software. The different expression of Pinl and mTOR protein in the two kinds of tissues were compaired by the paired x2test,We used the x2test to analyse the relationship of Pinl and mTOR protein with clinicopathological features in esophageal squamous cell carcinoma, the coefficient of contingency was used to analyse the correlation evaluation of two kinds of protein expression in esophageal squamous cell carcinoma, inspection level a=0.05.Results1. The expression of Pinl in normal esophageal tissues and esophageal squamous cell carcinoma, as well as the relationship with clinicopathological parameters.Positive Pinl protein expression in esophageal squamous cell carcinoma was60.0%(24/40), higher than the normal esophageal mucosa (32.5%,13/40), the difference was statistically significant (x2=4.762, P=0.027). Pinl expression with positive esophageal squamous cell carcinoma lymph node metastasis (P<0.05), and the degree of differentiation, TNM staging, age, gender (P>0.05). Pinl in esophageal squamous cell carcinoma, age less than or equal to55-year-old group the positive rate was54.2%(13/24), greater than55-year-old group, the positive rate was68.8%(11/16); of Pinl scales in the esophagus gender-shaped cell carcinoma of the male group, the positive rate was50.0%(11/22), the female group the positive rate was72.7%(13/18); of Pinl in esophageal squamous cell carcinoma in the TNM I-II group positive rate of70.0%(7/10) of III group, the positive rate of56.7%(17/30); of Pinl in the group of well-differentiated esophageal squamous cell carcinoma positive rate was50.0%(8/16),-of poorly differentiated group the positive rate was66.7%(16/24); of Pinl-positive group with lymph node metastasis in esophageal squamous cell carcinoma was77.3%(17/22), the positive rate of lymph node metastasis was38.9%(7/18).2. The expression of mTOR in normal esophageal tissues and esophageal squamous cell carcinoma, as well as the relationship with clinicopathologicalparametersMTOR protein positive expression in esophageal squamous cell carcinoma was62.5%(25/40), higher than the normal esophageal mucosa (27.5%,11/40), the difference was statistically significant (x2=6.036, P=0.014). The positive expression of mTOR with esophageal squamous cell carcinoma of the degree of differentiation, TNM stage, age, gender, independent of lymph node transfer showing no significant association (P>0.05). mTOR in esophageal squamous cell carcinoma aged less than or equal to55-year-old group was66.7%(16/24), greater than55-year-old group, the positive rate was56.3%(9/16); of mTOR in the esophagus scales gender-shaped cell carcinoma of the male group, the positive rate was63.6%(14/22), the female group the positive rate was61.6%(11/18); of mTOR in esophageal squamous cell carcinoma in the TNM I-II group positive rate was50.0%(5/10) of III group positive rate of66.7%(20/30); of mTOR in the group of well-differentiated esophageal squamous cell carcinoma positive rate was68.8%(11/16), in-of the poorly differentiated group the positive rate was58.3%(14/24); of mTOR positive lymph node metastasis in esophageal squamous cell carcinoma was68.2%(15/22), without lymph node metastasis-positive rate of55.6%(10/18).3. The relationship between Pinl and mTOR expression in esophageal squamous cell carcinoma. In esophageal squamous cell carcinoma at the same time19cases of mTOR expression was positive in24cases of Pinl expression positive group, while the remaining five cases of mTOR expression was negative;19cases of Pinl expression was positive in25cases of mTOR expression positive group, six cases of Pinl expression was negative, the correlation between the two expression were positively correlated (r=0.389, P=0.009).Conclusions1. Pinl in normal tissues have a low expression was highly expressed in esophageal squamous cell carcinoma and esophageal cancer, lymph node metastasis is closely related to the other.2. mTOR in normal tissues have a low expression, was highly expressed in esophageal squamous cell carcinoma and pathological parameters.3. Overexpression of Pinl and mTOR plays an important role in the development of esophageal cancer, the two interactions, induced the development of esophageal cancer.4. A positive correlation between Pinl and mTOR expression. The joint detection of Pinl and mTOR can improve the diagnosis, treatment guidance of esophageal cancer. |
PDF Full Text Request