Clinical Application Of Detecting IgH/IgK Gene Rearrangements In The Patients With B-cell Lymphoma

ObjectiveTo detect the IgH/IgK gene rearrangements in DNA from paraffin embedded tissues of the patients with B-cell lymphoma, then explore the application of IgH/IgK gene rearrangements in early diagnosis of B-cell lymphomas and the relationship with the clinical manifestation.MethodDNA was extracted from paraffin embedded tissues in50B-cell lymphomas (including33DLBCL,2small B-cell lymphoma,3Marginal zone lymphoma,5Follicular lymphoma,7Hodgkin lymphoma), and12lymphonode inflammation (negative controls). The IgH/IgK gene rearrangements were amplified by multiplex primer polymerase chain reaction (PCR), analyzed by double stranded heterologous for capillary electrophoresis and compared with results of negative controls. We discussed the relationship of IgH/IgK gene rearrangements with clinical manifestations (LDH level, β2-MG level, lymphoma stage, systemic symptoms). The results were analyzed by SPSS17.0statistics software.Results1. Totally45cases of B-cell lymphomas were positive for PCR rearragement to use heteroduplex technique detecting, while12cases of lymphonode inflammation were all negative(P<0.05).2.41patients(91.1%) with IgH/IgK gene rearrangements had higher serum LDH, while did less the patients(40%) without these gene rearrangements(P<0.05). None of lymphoma stage, systemic symptoms, β2.MG level, was significantly correlated with IgH/IgK gene rearrangements quality.ConclusionsThe IgH/IgK gene rearrangements might be used as specific indicator for diagnosis of B-cell lymphomas, and it might be related with serum LDH level.