Effect Of Highly Selective Nerve Injury On Skin Wound Healing In Rats

Objective To examine the effect of highly selective nerve injury on skin wound healing by establishment of a rat highly selective nerve injury combined with skin defect model.Methods A total of90male Sprague-Dawley rats were randomly divided into3different groups(30in each group), A group:posterior rhizotomy+cutaneous excision wounding group, B group:anterior rhizotomy+cutaneous excision wounding group, C group:sham operation+cutaneous excision wounding group. The healing rate of the wound was detected at2,7,14,and21days after injury; the CGRPmRNA expression was determined by Reverse transcription-polymerase chain reaction (RT-PCR) at1,3,7,and14days, and the bcl-2protien expression was deterimined by immunohistochemistry at3,7,14,and21days.Results All the rats surived at the end of experiment.The healing rate of the wound was significantly higher in group C than in groups A and B (P<0.05) at7,14,and21days. The expression of CGRPmRNA in3groups was observed by RT-PCR, CGRPmRNA expression was gradually up-regulated after wounding, the CGRPmRNA expression was significantly higher in groups B and C than group A (P<0.05) at1,3,and7days; showing significant difference both group B and group C (P<0.05) at7,and14days. Immunohistochemistry showed that the expression of bcl-2protien was observed in3groups, the bcl-2protien expression was significantly higher in group c than in groups A and B(P<0.05) at 3,7,and14days, showing significant difference both group A and group B (P<0.05) at14,and21days.Conclusion In wound healing process, nerve injury can delay wound healing, the effect of sensory nerve to the healing process is more significant than the motor nerve. Denervation of the nerves(especially the sensory nerves)is not conducive to wound healing.Therefore, a complete innervation is an indispensable condition for normal wound healing.