The Relationship Between ICAM-1, MCP-1Plasma Level And Gene Polymorphisms With The Susceptibility Of Enterovirus71Infection In Hand, Foot And Mouth Disease

Objective To study the relationship between ICAM-1, MCP-1plasma level and gene polymorphisms with the susceptibility of enterovirus71infection in Hand, Foot and Mouth Disease.Methods By reverse transcription polymerase chain reaction(RT-PCR), we made use of EV71specific primer to detect enterovirus71in the serum of Hand, Foot and Mouth Disease (HFMD) children. PCR—RFLP techniques and direct sequencing were performed to determine the genotypes of1CAM-1K469E and MCP-1A-2518G gene in EV71infection group and in normal group. The plasma levels of sICAM-1and MCP-1were detected by enzyme-linked immunosorbent assay.Results Compared with the normal children, the plasma levels of sICAM-1and MCP-1of the patients were significantly increased(P<0.01). In EV71infection group, A allele of ICAM-1K469E were less common than in the normal group (X2=6.897, P<0.01). There were no significant differences in the distribution of frequency of MCP-1A-2518G genotype and allele frequency between the EV71group and the normal group(P>0.05).Conclusion Plasma level of sICAM-1expression and the polymorphism of ICAM-1K469E single nucleotide may corelate with EV71infection.A allele of ICAM-1K469E may reduce the incidence of enterovirus71infection in hand, foot and mouth disease.Plasma level of MCP-1expression may corelate with EV71infection,but no marked association could be found between A/G polymorphism in MCP-1A-2518G gene and EV71infection.