The Study On Mechanism Of Promoting Gastrointestinal Peristalsis In Mice By Curcumin

Background:Our previous research has showed that the pre-administration of curcumin can attenuate the gastrointestinal injury caused by cisplatin, promote gastrointestinal peristalsis. But the precise mechanism is not elucidated at present. At this study, we will discuss the concrete of curcumin’s promoting gastrointestinal peristalsis from the two aspects:the effect of activating M-acetylcholine receptors and the effect of inhibiting NO who is the inhibitory neurotransmitter of NANC nerve, to establish the experimental basis for clinical application of curcumin.Objective:to study the mechanism of promoting gastrointestinal peristalsis by curcumin and to establish the experimental basis for clinical application of curcumin.Methods:Forty-eight three-week-old male Kunming mice, which were bought from Qingdao Institute for Drug Control, were randomly divided into six groups:control group, atropine group, curcumin group, atropine+curcumin group, L-Arg group and L-arg+curcumin group.The mice in normal control, atropine and L-arg groups were orally administered with0.2ml solvent solution (5%Arabic gel) once a day for15times, the mice in curcumin group, atropine+curcumin group and L-arg+curcumin group were orally administered with0.2ml curcumin (200mg·kg-1) in solvent solution (5%Arabic gel) once a day for15times. On the11th day, the mice in L-arg, L-arg+curcumin group were given L-arg by administered intragastrically with (2mg·kg-1,0.2ml, once a day for5times).At the same time, the mice in normal control were given saline by administered intragastrically with in the same way.On the16th day, no food was supplied to mice while water could be drunk freely for24hours. The mice in atropine and atropine+curcumin group were given atropine by intraperitoneal injection (0.5mg·kg-1,0.2ml)20minutes before the experiment. The mice in all groups were orally administered with black ink in solvent solution(0.2ml). After20minutes, the mice were killed by dislocated death. At laparotomy, isolation of gastrointestinal tissue was conducted and tissue was laid flat, maintaining tensionless. The length of small intestine and the length of ink progradation in it were calculated. The motional rate of intestine was calculated by:the distance of ink in intestine from pylorus/the overall length of intestine×100%. Results:In every group there was no significant difference in weight (P>0.05) before and after intragastric administration and medication. The motional rate of small intestine in curcumin group was not obviously different from that in control group(P>0.05. The motional rate of small intestine was significantly decreased in atropine group (P<0.01) and L-arginine group(P<0.01), but it was significantly increased in curcumin+atropin group (P<0.01) and curcumin+L-arginine group (P<0.01), compared with that in atropine group and L-arginine group.Conclusion:The mechanism of promoting gastrointestinal peristalsis by curcumin maybe by activating M-receptor of Ach and inhibiting the action of NO, which is inhibitory neurotransmitter Of NANC nerve.Conclusion:1. Curcumin has no effect on normal gastrointestinal motility of mice.2. Curcumin can significantly improve the gastrointestine motility induced by Atropine and L-arginine.3. The effects of curcumin improving the gastrointestine motility may play by affecting M-receptor of acetylcholine pathway and NO pathway.