Abnormal Expression Of Annexin A2 Regulated By Ubiquitination Contributed To Pathological Process Of Breast Cancer

Objective：To study effects of abnormal annexin A2 on breast cancer byobserving regulation of ubiquitination by annexin A2 and different expressionof annexin A2 from the clinical different stage of breast cancer.Materials and Methods:1. Materials: Ten groups of fresh breast cancer and adjacent normaltissues (two samples from T1N0M0 stage, four from T2N0M0, one fromT2N1M1 and three from T3N1M0) were obtained after surgical mastectomyfrom female patients. Meantime, twenty-eight paraffin samples of humanbeing breast cancer were got from the Department of Pathology in theAffiliated Hospital of North Sichuan Medical College. All samples wereidentified as infiltrating ductal carcinoma.2. Methods: After tissue protein prepared, western blotting analysis wasused to observe expression and ubiquitination of annexin A2 withanti-ubiquitin antibody. Then, after 2-DE, immunoproteomics with PDQuestsoftware (7.1), MALDI-TOF-TOF MS/MS and bioinformatics analysis wasperformed to obtain differential proteins ubiquitinated. Different expressionof annexin A2 from the clinical different stage of breast cancer was observedand analyzed with immunohistochemical staining and SPSS13.0.Results:By western blotting, there was an obvious differential strap withmolecular weight of approximately 45kDa) on membranes while there was same molecular weight protein spot on membranes after 2-DE.Four proteins were identified by immunoproteomics, that is, SMT3A,PSMA1, annexin A2 and RPS12. By immunohistochemical staining analysis,positive expression rates of annexin A2 in breast cancer and the adjacentnormal tissues were respectively 90% (9/10) and 40% (4/10) (P < 0.05),Meantime, in zero,Ⅰ,ⅡandⅢstages, positive rates of annexin A2 wererespectively 75％, 100％, 100％and 100％while strong positive rates wererespectively 37.5％, 42.9％, 50％and 66.7％(P<0．05).Conclusion：Annexin A2 was over-expressed in breast cancer tissues andrelated with invasion and metastasis of breast cancer cell; annexin A2 wasregulated by ubiquitination; more later clinical stages of TNM, more higherexpression of annexin A2; over-expression of annexin A2 regulated byubiquitination may be involve in breast cancer; there may be a new strategyfor therapy of patients with breast cancer that annexin A2 and itsubiquitination were clinically interfere in.