Glucose And Lipid Metabolism Regulated By SARS-CoV N Protein The Interaction With AHSG In Mice

SARS is the severe acute respiratory syndrome, the first case occurred in Guangdong, China on December16,2002. Soon after this it spread in the mainland and Hong Kong, at last around the world. SARS is respiratory disease of the respiratory infections that is mainly caused by a new type of coronavirus--severe acute respiratory syndrome coronavirus(SARS-CoV). SARS patients who are infected, appear the fever, myalgia and other systemic symptoms, there will be significantly higher of the blood glucose and lipid, resulting in causing the patients which have a history of the diabetes sicker and increased mortality. Although from2004that did not occur SARS infection between people, we can not completely rule out the possibility of SARS broke out again, because the studies have found that in bats and civets and other animals continue to find a similar virus. In recently years, SARS epidemic regularity at home and abroad, pathological characteristics, treatment and prevention and control measures of drugs and vaccines and done a lot of research and made a series of research progress, but about the SARS pathogenesis mechanism, there are still many unknown domain, which still need continue to explore and research.SARS-CoV N protein is the second largest in the SARS-CoV protein structure, which can be formed nuclear capsid with virus RNA. SARS-CoV N protein is made up by422amino acid residues composition, biophysics research shows that SARS-CoV N protein consists of two independent structure domain and a link to a region. SARS-CoV N protein is in a virus particles core part, which is main component of nuclear capsid structure, with its genome RNA-binding complex formation, this to viral genome sequence identification, RNA virus particles assembly have an important role. SARS-CoV N protein is the main immune response the original, can induce strong humoral immune and immune cells. Early in the virus infection, SARS-CoV N protein can stimulate the body to produce antibody, which can be confirmed from acute early recovery of the patients’serum, therefore probably it can detect a virus infection in the earlier, which has an important role to early diagnosis and prevention of the SARS. Tire globulin A is whole called a2-Heremans-Schmid glycoprotein, which is abbreviated for AHSG. AHSG protein is a kind of synthesized by the liver of important plasma protein, which is transmitted to the whole body through the blood circulation, then gathered in mineralization bones and teeth, involved in the body’s biological reaction. AHSG also is a kind of natural against stimulated insulin receptor tyrosine kinase (IRTK) inhibitory factor, which can restrain the body specifically in vitro insulin or excite insulin receptor (IR) phosphorylation itself, also can inhibit the exogenous substrates of tyrosine phosphorylation, will eventually blocking insulin signaling pathways conduction. Second AHSG to maintain body fluids of calcium ion concentration and the suppressing organization cells calcification has an important role.The early research of our lab shows that, directly interaction exist SARS-CoV N protein and AHSG, which through the AHSG mediated inhibit insulin signaling pathways, affecting the body’s glucolipid metabolism. In order to further validation SARS-CoV N protein on the body glucolipid metabolism is the effect of AHSG mediated through, this study AHSG gene knock out mice and the C57C57wild type mice as the research object, the SARS-CoV N protein on lipid metabolism of mice sugar to glucose, the use and remove the ability and the influence of insulin sensitiity. At the same time from the cell level and mice level discusses SARS-CoV N protein on the lipid synthesis genes FAS, SREBP-1, the influence of the expression level.