Established A Training Platform Of Para-carcinoma Tissues In Vitro To Study Berberine Inhibit Colorectal Carcinoma Activities

Object iveCulturing the organization form the different parts of colorectal carcinoma to investigate the culture conditions of human congress bowel tissue in vitro and the time of maintaining the complete crypt structure under the different conditions. Using the medium containing berberine to culturing the organizations form the different parts of colorectal carcinoma for the aim to investigate how to maintain the stability of the crypt and treatment and prevention of colorectal carcinoma.MethodsObtaining surgical specimens from the place about2cm,5cm or10cm next to tumors,and then training the specimens by the way of repeating stick wall.Observationing the growth of organization,to take a picture on the3rd, the7th,the14th and the20th day.removing tissues to cut into slices,stained by HE. Training organization biopsy of large intestine cancer different parts by using respectively the Serum-free medium and the serum-free medium including105μmol/L berberine in3days, removing tissues to cut into slices,stained by HE,IHC.ResultsBy observing the growth situation about the large intestine cancer tissues in vitro,we find that the tissues have the highest survival rate,the closer the tumors,the growth activity is higher.In the medium containing serum,along with the serum concentration increases.the growth of the cell is restrained.From the observing of the stained by HE,we seen the Whether serum-free culture or contain serum training,the situation about the crypt of The tissues did not chang significantly in3days. But after7days, the cells would be gradually reduced in the crypts, and even apart. Training organization biopsy of large intestine cancer different parts by using respectively Dkkl medium and the serum-free medium including105U mol/L berberine in3days and7days, we found the color of the cytoplasm, cell membrane and nuclei is same,the dyed cells is not obviously different to by using the serum-free medium including105μmol/L berberine or by using Dkk1medium. This did not suggest that berberine could restrain the excessive expression of β-catenin in the nucleus.ConclusionsThe survival time of humen intestinal tissues are negative correlated with the serum concentrations.the time of maintaining the structure of the crypt are negative correlated with the serum concentrations. This did not suggest that berberine could restrain the excessive expression of β-catenin in the nucleus and by this it can inhibition of the abnormal proliferation of the crypt,make the structure of the crypt maintain intact and treatment and prevention of colorectal carcinoma.