The Levels And Significance Of Plasma PLGF And S100A12in Patients With End-stage Disease

Objective:To study the levels and clinical significance of plasma PLGF and S100A12in patientswith CKD5.Methods:We measured plasma PLGF and S100A12concentration in67CKD5.The patientswere divided into cardiovascular and cerebrovascular diseases(CVD) and without CVDaccording to cardiovascular complication. The patients were divided into nondialysis(non-HD)and hemodialysis(HD) according to the therapies.There were26selecetedpatients with nondialysis in total,including14male,12females,the average age was (53.69±14.31) years.There were41seleceted patients with hemodialysis in total,including30male,11females,the average age was (59.05±17.83) years.The patients were divided intodifferent groups according to cardiovascular complications:①non-HD with CVD group:5patients;②non-HD without CVD group:21patients;③HD with CVD group:18patients;④HD without CVD group:23patients.Twenty sex-and age-matched healthy chinesevolunteers were recruited as normal controls.Concentrations of PLGF and S100A12inplasma were measured by Enzyme linked immunosorbent essay (ELISA).And the clinicaland laboratory data of the patients were collected.Results:1. Plasma PLGF levels of patients with CKD5were significantly higher than controlgroup (P<0.01),and with CKD5and CVD were not higher than without CVD(P<0.01).2.Plasma PLGF levels of patients with HD were significantly higher than control groupand non-HD group(P<0.01,P<0.01);there were no significant difference in PLGF levalbetween non-HD group and control group(P＞0.05).3. In non-HD group,plasma PLGF levels were higher in patients with CVD than thosewithout CVD (P＜0.01)； in HD group,plasma PLGF levels were higher in patients withCVD as compared to without CVD (P＜0.05). 4. In HD group and CKD5group,plasma PLGF levels were positive correlated withserum total cholesterol (P＜0.05).In non-HD group,plasma PLGF levels were positivecorrelated with serum total cholesterol and intima-media thickness (P＜0.05).5. Plasma S100A12levels of patients with CKD5were significantly higher than controlgroup (P<0.01),and with CKD5and CVD were not higher than without CVD(P<0.01).6.Plasma S100A12levels of patients with HD were significantly higher than controlgroup and non-HD group(P<0.01);plasma S100A12levels of patients with non-HD werehigher than control group(P<0.01).7. In non-HD group,plasma S100A12levels were higher in patients with CVD thanthose without CVD (P＜0.01)； in HD group,plasma S100A12levels were higher inpatients with CVD as compared to without CVD(P＜0.01).8. In HD group and CKD5group, plasma S100A12levels were positive correlated withserum white blood cell,neutrophil percentage,high-sensitivity C-reactive protein (P＜0.05),and negatively correlated with albumin (P＜0.05).In non-HD group, plasma S100A12levelswere positive correlated with neutrophil percentage,high-sensitivity C-reactiveprotein,glucose,IMT (P＜0.05),and negatively correlated with albumin (P＜0.05).9.In CKD5group、 HD group and non-HD group,plasma PLGF levels weresignificantly positive correlated with plasma S100A12levels(P＜0.01,P＜0.01,P<0.01).Conclusions:Plasma PLGF and S100A12levels are elevated in patients with CKD5,and thiselevation is significantly in patients with HD.In HD group and non-HD group,plasmaPLGF and S100A12levels are significantly elevated in patients with CVD than thosewithout CVD.In non-HD group, plasma PLGF and S100A12levels positive correlatedwith carotid endarterectomy thickness.All of the above suggested that in CKD5patients,plasma PLGF and S100A12may be involved in the pathophysiology of CVD.InCKD5patients,especially in HD patients.