| Objective: After the rats with focal cerebral ischemia-reperfusion injury are treated with rhG-CSF and citicoline, observe the Bcl-2and Bax ’s expression, clear the cerebral protective effect of rhG-CSF and citicoline on rat brain after focal cerebral ischemia-reperfusion injury and explore its mechanism of cerebral protective effect.Methods:72adult male Wistar rats (230±10g),which were divided into model group (n=18), rhG-CSF group (n=18), citicoline group (n=18), rhG-CSF combined with citicoline group (n=18) randomly,. Each group was subdivided into6h,24h,72h three subgroups. Prepare the rats model of focal ischemia-reperfusion by modified Zea Longa method, detection of neuronal apoptosis with TdT-Mediated dUTP Nick-End Labeling(TUNEL), observe the pathological changes of the brain tissue form (HE staining), determine the average optical density value of Bax and Bcl-2at different times in rats brain after focal cerebral ischemia-reperfusion using immunohistochemical method.Results:HE staining showed that the neuronal injury is significantly reduced after treated, compared with model group,the combined group for the most,Immunohistochemistry showed that in all treated groups the expression of Bcl-2increased and Bax reduced compared with model group,24h reached the most.the combined group Bcl-2protein expression at most, Bax positive expression at least at all time points(P<0.05).TUNEL staining showed that the model group surgery contralateral cerebral hemisphere occasionally apoptotic cells and the lesion shows a large number of apoptotic cells (P<0.05); citicoline treatment group and rhG-CSF treatment group the number of apoptotic cells are significantly reduced,the number of apoptotic cells in the combined treatment groupat least (P<0.05).Conclusion:HE staining showed that rhG-CSF and citicoline treatment have eased at different time points in neurological deficit and infarct size compared with the model group, the combined treatment group at the most.Through reducing cerebral ischemia and reperfusion after nerve injury,rhG-CSF and citicoline achieve neuroprotective effect. In the model group, the hippocampus and cortex can be seen a large number of apoptotic cells, which can significantly improve after rhG-CSF and citicoline treatment. rhG-CSF, Citicoline treatment increases cerebral ischemia and reperfusion injury in rat Bcl-2expression, reducing Bax expression, especially combination therapy is more effective, effective prevention and treatment of cerebral ischemia and reperfusion injury. |
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