Expression Of Tumor Stem Cell Marker CD44v6 And CD133 In Laryngeal Squamous Cell Carcinoma

Objective: To investigate the expression and clinical significance of tumor stem cell markerCD44v6 and CD133 in laryngeal squamous cell carcinomas. To analysis thereasonableness for CD44v6 and CD133 as the Tumor Stem Cell Marker of laryngealsquamous cell carcinoma.Methods: The clinical data from 53 primary laryngeal squamous cell carcinomas patients wereretrospectively analyzed. Selected 53 cases of primary laryngeal squamous cellcarcinomas tissue paraffin block of which case data complete, collected 20 cases ofnormal tissue and cultured for 3 groups of hep-2 cells for experimentalresearch.Immunohistochemical staining was used to detect the expression of CD44v6and CD133 in 53 cases of patients with laryngeal squamous cell carcinomas, 20 casesof normal tissue and hep-2 cell. SPSS 17.0 statistical software was used for statisticalanalysis, using the Chi-square test and Fisher’s exact test compared with the differentexpression of CD44v6 and CD133 protein in laryngeal carcinoma with differentclinical type, different T, N stages and with different degree of differentiation,inspection levelα= 0.05, P < 0.05 for the difference was considered statisticallysignificant. The correlation between CD44v6 and CD133 protein analysis usingSpearman rank correlation analysis, P < 0.05 was considered statistically significant.Results: The positive rates of CD44v6 protein in laryngeal cancer tissue and normal laryngealmucosa tissue were 75.47% and 30%, there was significant difference (P < 0.05); Thepositive rates of CD133 protein in laryngeal cancer tissue and normal laryngealmucosa tissue were 71.69% and 25%, there was significant difference ( P < 0.05).Thepositive rates of CD44v6 expression was relate to histological differentiation and Tstage (P<0.05). CD44v6 protein expression were not correlated with staging andlymph node metastasis (P>0.05). The positive rates of CD133 expression was relate tohistological differentiation, T stage and lymph node metastasis (P<0.05). CD133protein expression were not correlated with staging (P>0.05).The mean positive ratesof CD44v6 in Hep-2 cell are 74.3%, the mean positive rates of CD133 in Hep-2 cellare 2.70%. Conclusions: The positive rate of CD44v6 in laryngeal cancer is the same as in hep-2 cell, thepositive rate of CD133 in hep-2 cell is low. The expression of CD44 and CD133may play an important role in the progression of laryngeal cancer. There is acorrelation between CD44v6 and CD133 protein expression in laryngealcarcinoma and some malignant characteristics, more studies are needed to answerthe question that CD44v6 and CD133 are the Tumor Stem Cell Marker oflaryngeal cancer, more studies are needed to answer the question that CD44v6 andCD133 are the Tumor Stem Cell Marker of laryngeal cancer.