| Autologous cartilage transplantation is currently the cosmetic work most commonly means, cartilage transplantation in improve areas at the same time, the donor area of the physiological function, appearance will cause different degree injury. Long-term since, for the area of secondary lesions, progressive for structural abnormalities, graft early absorption, limited available cartilage volume and cartilage grafts and recipient with poor ability, is in the main disadvantages of autograft cartilage transplantation.Therefore, how to correct the deformity at the same time, avoided to the greatest extent of the zone of injury and loss of supply, increase the use of tissue volume has been one of the problems facing clinicians. Regeneration of cartilage and perichondrium cell factor in all the action in the process has attracted a lot of attention.ObjectiveThe discussion to the collagen sponge as a carrier, combined with recombinant human bone morphogenetic protein2(rhBMP-2) implantation in rabbit ear cartilage defects in vivo induced cartilage membrane cartilage regeneration effect. MethodsSelection of healthy adult9Japanese rabbits, male and female, weight about2.5kg. Randomly divided into groups, rabbit collagen sponge containing0.5mg/ml rhBMP-2/collagen sponge composite, blank control group group three. Xylazine hydrochloride injection intramuscular anesthesia (0.2mL/kg), two ears with Iodophor disinfection, in each side from the ears of5cm rabbit ear side and rear, avoid vascular Meilan mark the cut line and lcm x lcm cartilage resection area. The amount of0.5%lidocaine injection in postoperative of subcutaneous tumescent anesthesia, along the line of incision skin incision, carefully open the perichondrium, avoid damage to cartilage, with periosteal stripping ion in cartilage membrane under careful separation, removal of lcm x lcm cartilage, intact cartilage membrane. Group A (8ears):the cartilage defect with implant and removal of cartilage of equal size, the thickness of the collagen sponge in cartilage membrane. Group B (8ears):the cartilage defect with implant and removal of cartilage thickness of equal size, containing0.5mg/mL concentration of rhBMP-2/collagen sponge composite for cartilage membrane. Group C (8ears):blank control group, not implanted in any material. Operation interrupted suturing, wound with mupirocin ointment. After4,8,12weeks in animal death, material, removal of cartilage membrane were observed in cartilage, area; by HE staining, Masson staining and aldehyde fuchsin staining, specimens were observed in cell invasion and differentiation of cartilage growth, and elastic fiber changes and formation.Results1.General observation:Postoperative1--4week good wound healing, the operation had no significant difference, and the normal cartilage boundaries are clear. C group afterl weeks,2of4repair region forming a black eschar, necrosis,4weeks postoperatively decrustation perforations are formed; after8weeks in the A group operation area and surrounding normal cartilage boundaries are still recognizable, B group and normal cartilage boundaries are not clear; after12weeks, the A group formation the new organization of color, texture and12week closed, part of the specimen and the surrounding normal cartilage boundaries are still legible. B group and normal cartilage boundaries disappear, color, texture and12weeks near normal cartilage, and similar.2.Histological observation:After4weeks, each group had no new elastic fibers. A group within cartilage matrix cell infiltration, chondrocyte distribution is more uniform, arranged without direction. Group B cartilage membrane thickening, cartilage matrix cell infiltration, visible cartilage cell infiltration into the collagen material structure. Group C cartilage membrane thickening, cell number, with a large number of fibroblasts, also have a typical cartilage cells, inflammatory cells are common;8weeks after operation, no defect in collagen sponge. A group within cartilage matrix cell infiltration is little, still have inflammatory cell infiltration. Compared with normal cartilage at the junction of the connection is still not complete. Group B cartilage thinning of the film, has the rare or not, have a typical cartilage cells appear, see a few new elastic fibers. Group C perichondrial cells was significantly decreased at4weeks, cartilage cells more visible, neovascularization, inflammatory cells disappeared;12weeks after operation:A group than the normal cartilage cartilage slightly thin, cellular derangement, matrix metachromatic staining pale, with normal cartilage has not yet fully connected, see a few new elastic fibers. Group B cartilage near normal cartilage surface cells, arranged in parallel, deep arrangement is disorder, matrix metachromasia, compared with normal cartilage good connection, see a large number of new elastic fibers. Group C defect edge and bottom form a small amount of cartilage cell, uneven distribution. Using a blinded to the specimens for histological score, score standards by Wakitanifa method [1]. In A group, B group and C group in each period, there were significant differences were statistically significant (P<0.01). In two experiments A, B group4,8,12weeks had a significant difference (P<0.01), at the same time in two experimental groups showed no significant differences (P<0.05).3. Determination of the area of new cartilage:HPIAS-1000high-definition color pathological image analyzer the area of new cartilage, in pixels. All specimens complete removal of the cartilage exposed cartilage parallel sections after HE staining, determination of new cartilage in between the cartilage area. Collagen sponge group, rhBMP-2/collagen sponge complex group of pixels of blank control group were1452±549.8,1962±524.4,1197±496.2. The analysis of variance, the three groups resulting in significant difference was statistically significant (P<0.05).Conclusions1.rhBMP-2can promote to promote cartilage at the cartilage regeneration.2. collagen sponges as rhBMP-2is safe and effective carrier, at the same time provide the conditions for cartilage regeneration of cartilage.3.suggesting that the collagen sponge to the role of cartilage regeneration.4. the application of the two complexes for cartilage cut after surgery to repair the cartilage defect to provide a new approach. |
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