Expression Of Transcription Factor NKX2-1in The Colon Of Patients With Hirschsprung’s Disease(HD)

Background and ObjectiveCongenital megacolon (Hirschsprung’s disease, HD)is a relatively common digestive tract malformation and a congenital disease.Incidence of the disease is approximately1/5000. Colon bowel thickening is one of the reasons. It may occur in the proximal colon, sigmoid colon and general as part of the descending colon and may also in expand the distal intestine, rectum and sigmoid colon is the part; part two is arranged between the transition zone or transitional zone, often forms a funnel shape,3cm-8cm long., It has been confirmed that NKX2-1throughout the development of the nervous system in existence.The gene is located on chromosome14q13,its encoded protein products containing371amino acid residues, a molecular weight of38000, contains3exons and introns, is a containing helix turn helix structure of transcription factors. In recent years, NKX2-1’s research is mainly about the thyroid, brain and lung disease related to nervous system development process research, in the enteric nervous system research is less. Congenital giant colon intestinal peristalsis may be associated with transcription factor NKX2-1in children with HD expression in intestinal wall have a certain degree of relevance, this experiment is using immunohistochemistry and RT-PCR technique for detection of NKX2-1in HD colon wall in expression, further understanding of the HD in the molecular basis of pathogenesis to provide reference for the clinical diagnosis. Materials and Methods1To observe the expression of Transcription factor NKX2-1in surgical specimens of30case of HD (narrow segments、transition segments and Dilation segments in separate) and of30cases of normal colon tissue with the method of immunohistochemistry.2To observe the expression of Transcription factor NKX2-1in surgical specimens of30case of HD (narrow segments、transition segments and Dilation segments in separate) and of30cases of normal colon tissue with the method of RT-PCR3Statistical analysis:data applications SPSS17.0software processing, transcription factors in colon tissue of the colon in each group and normal control group, the HD children of NKX2-1protein expression was used to compare two independent samples rank sum test; HD children colon in each groupand normal colon tissue of the control group transcription factor of NKX2-1mRNA expression was used to compare two independent samples t-test or calibration t-test. Inspection level a=0.05.Results1Transcription factor NKX2-1is apparently expressed less in narrow segments of HD and normally in dilation segments, the expression of the Transcription factor NKX2-1in transition segments between the two.2The protein expression of Transcription factor NKX2-1of narrow and transition segments in HD colon tissue and normal colon tissue were singnificantly different(Z=5.236and2.926,P<0.008; t’=19.475and14.429,P<0.05),The protein expression of Transcription factor NKX2-1of dilationsegments in HD colon tissue and normal colon tissue were no singnificantly different(Z=2.336,P=0.019>0.008; t=0.985,F=0.329>0.05)。Conclusion1Like the absence of ganglion cells,the less expression of the Transcription factor NKX2-1contributes to the pathological changes of HD.2The reduction of transcription factor of NKX2-1expression in the intestinal wall of Hirschsprung is possibly one of the reasons that lead to HD onset.