The Relationship Between The Genepolymorphism Of TGF-β1Gene And The Infection Of HBV As Well As The Clustering Of Hepatocellular Carcinoma In Families In Guangxi

Objective:To investigate the relationship between rs1800469single nucleotide polymorphism (SNP) of the TGF-(31gene and the infection of HBV as well as the clustering of hepatocellular carcinoma in the families in Guangxi, China.Methods:Blood samples collected from114family members (FHCC) whose families have had two or even more hepatocellular carcinoma patients and114healthy controls(FNC) that match the same nationality, gender, HBsAg and about±5years of age who come from the families without any cancer. Single nucleotide polymorphism (SNP) at loci of rs1800469such as genotypes(CC,TC,TT) and alleles(T,C) were analyzed by using DNA sequence and then through polymerase chain reaction-restriction fragment length polymorphism methods.Results:(1) The distribution of genotypes(CC,TC,TT) at loci of rs1800469was according with the Hardy-weinberg equilibrium (P>0.03).(2)There was no statistical differences observed between FHCC group and FNC group in the frequencies of the alleles(T,C) and genotypes(CC,TC,TT) at loci of rs1800469(P>0.05).(3) There was no significant difference in the distribution of allelics(T,C) and genotypes (TC,TT) frequency between the subjects who have infected with chronic hepatitis B (HBsAg positive) and noninfected(HBsAg negative)(P>0.05). However, there was a statistical differences in genotype CC between HBsAg positive group and HBsAg negatine group (P<0.05).Conclusions:(1) The distribution of genotypes(CC,TC,TT) at loci of rs1800469was according with the Hardy-weinberg equilibrium, so there was no sample seletion bias and this study selection who came from the general population could has the group representative.(2)The alleles (T,C) and genotypes(CC,TC,TT) at loci of rs1800469in TGF-β1gene were non-associated with the clustering of hepatocellular carcinoma in families in Guangxi province.(3) At loci of rs1800469in TGF-β1gene, the genotype CC might increase the risk for the infection of chronic hepatitis B virus, while the allelics(T,C) and genotypes(TC,TT) had no effect for that.