Effect Of Intravenous Immuno Globulin On Function Of Cord Blood Helper T Cells In Preterm Infants

Objective To investigate the immunoregulating effects of different doses intravenous immunoglobulin(IVIG)on helper Tcells(Th)in cord blood of preterm infants and to provide the experimental bases for correct clinical use IVIG.Methods Cord blood mononuclear cells(CBMCs)were isolated by density gradient centrifugation method from15preterm infants with gestational age32-34weeks,which were divided into3groups(Group A,6mg/ml;Group B,9mg/ml;Group C,12mg/ml)according to different concentrations of IVIG,then cultured for72hours in sterile conditions.In the end,the levels of IFN-y and IL-4in the supernatant were determined by enzyme-linked immunosorbent assay(ELISA).Results(1)The level of IFN-y in Group C(32.59±26.82pg/ml)was much less than that in Group A(175.68±86.82pg/ml)and Group B(124.51±56.06pg/ml)(P<0.01),while compared with Group A(175.68±86.82pg/ml),the level of IFN-y in Group B(124.51±56.06pg/ml)did not decrease significantly(P>0.05).(2)Compared with Group A(29.04±8.27pg/ml),the level of IL-4in Group B(18.01±5.72pg/ml)and Group C(14.44±5.18pg/ml)decreased significantly(P<0.01),while the level of IL-4in Group B(18.01±5.72pg/ml)had no significant difference with that in Group C(14.44±5.18pg/ml)(P>0.05).(3)Compared with Group A(5.61±2.38)and Group B(8.22±4.21),the ratio of IFN-y/IL-4in Group C(2.41±1.72)decreased significantly(P<0.01).There was also significant difference between Group A and Group B(P<0.05).Conclusions IVIG can inhibit the function of Th cells in CBMCs of preterm infants,which was related with the concentration of IVIG.Low doses of IVIG can inhibit the function of Th2mainly;Large doses of IVIG can inhibit the function of Thl and Th2at the same time,but mainly Thl.